1887

Abstract

, the causative agent of bubonic plague, emerged recently (<20000 years ago) as a clone of . There is scant evidence of genome diversity in , although it is possible to differentiate three biovars (antiqua, mediaevalis or orientalis) based on two biochemical tests. There are a few examples of restriction fragment length polymorphisms (RFLPs) within ; however, their genetic basis is poorly understood. In this study, six difference regions (DFRs) were identified in , by using subtractive hybridization, which ranged from 46 to 19 kb in size. Four of the DFRs are flanked by insertion sequences, and their sequences show similarity to bacterial genes encoding proteins for flagellar synthesis, ABC transport, insect toxicity and bacteriophage functions. The presence or absence of these DFRs (termed the DFR profile) was demonstrated in 78 geographically diverse strains of . Significant genome plasticity was observed among these strains and suggests the acquisition and deletion of these DNA regions during the recent evolution of . biovar orientalis possesses DFR profiles that are different from antiqua and mediaevalis biovars, reflecting the recent origins of this biovar. Whereas some DFR profiles are specific for antiqua and mediaevalis, some DFR profiles are shared by both biovars. Furthermore, the progenitor of , . (an enteric pathogen), possesses its own DFR profile. The DFR profiles detailed here demonstrate genome plasticity within , and they imply evolutionary relationships among the three biovars of , as well as between and . .

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2002-06-01
2019-12-14
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