1887

Abstract

When is subjected to phosphate starvation, the Pho and σ-dependent general stress regulons are activated to elicit, respectively, specific and non-specific responses to this nutrient-limitation stress. A set of isogenic mutants, with a β-galactosidase reporter gene transcriptionally fused to the inactivated target gene, was used to identify genes of unknown function that are induced or repressed under phosphate limitation. Nine phosphate-starvation-induced () genes were identified: , , and were regulated by the PhoP–PhoR two-component system responsible for controlling the expression of genes in the Pho regulon, while (renamed ), , , and were dependent on the alternative sigma factor σ, which controls the expression of the general stress genes. Genes and are unique members of the Pho regulon, since they are phosphate-starvation induced via PhoP–PhoR from a sporulation-specific σ promoter or a promoter that requires the product of a σ-dependent gene. Null mutations in key regulatory genes and showed that the Pho and σ-dependent general stress regulons of interact to modulate the levels at which each are activated.

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2002-05-01
2019-11-13
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