RT Journal Article SR Electronic(1) A1 Krin, Evelyne A1 Sismeiro, Odile A1 Danchin, Antoine A1 Bertin, Philippe NYR 2002 T1 The regulation of Enzyme IIAGlc expression controls adenylate cyclase activity in Escherichia coli JF Microbiology, VO 148 IS 5 SP 1553 OP 1559 DO https://doi.org/10.1099/00221287-148-5-1553 PB Microbiology Society, SN 1465-2080, AB During the last few years, several genes, such as pap, bgl and flhDC, have been shown to be coregulated by the histone-like nucleoid-structuring (H-NS) protein and the cyclic AMP-catabolite activator protein (cAMP/CAP) complex, suggesting an interaction between both systems in the control of some cellular functions. In this study, the possible effect of H-NS on the cAMP level was investigated. In a CAP-deficient strain, the presence of an hns mutation results in a strong reduction in the amount of cAMP, due to a decrease in adenylate cyclase activity. This is caused by the reduced expression of crr, which encodes the Enzyme IIAGlc of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS), from its specific P2 promoter. This leads to a twofold reduction in the global amount of Enzyme IIAGlc, the adenylate cyclase activator, responsible for the decrease in adenylate cyclase activity observed in the hns crp strain., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-5-1553