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Volume 148, Issue 5

The genetic structure of populations in primary and secondary habitats Free

Abstract

were recovered from the members of two two-person households and their associated septic tanks. The were isolated using selective and non-selective isolation techniques, characterized using the method of multi-locus enzyme electrophoresis and screened for the presence of virulence factors associated with extra-intestinal disease by using PCR. The growth rate–temperature relationships of strains from the two habitats were also determined. Temporal variation explained 25% of the observed electrophoretic type (ET) diversity in the humans. Among-host variation accounted for 29% of the observed allelic diversity. In one household, ET diversity of the population in the septic tank was significantly lower than ET diversity in the humans providing the inputs to the septic tank. Molecular analysis of variance revealed that, on average, strains recovered from the septic tank of this household were genetically distinct from strains recovered from the humans providing the faecal inputs to the septic tank. Further, the growth rate–temperature response of strains differed between strains isolated from the septic tank and strains isolated from the humans. Septic tank isolates grew better at low temperatures than strains isolated from humans, but more slowly at high temperatures compared to the human isolates. By contrast, no real differences in ET diversity, allelic diversity, or the growth charcteristics of strains could be detected between strains from the humans and strains from the septic tank of the other household. The results of this study suggest there are strains of that are better ‘adapted’ to conditions found in the external environment compared to strains isolated from the gastrointestinal habitat. Further, the finding that the numerically dominant clones and clonal diversity in secondary habitats can differ substantially from those found in the source populations will confound efforts to identify the sources of faecal pollution in the environment.

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Keyword(s): amova, molecular analysis of variance , coliforms , ET, electrophoretic type , human , MLEE, multi-locus enzyme electrophoresis , population structure and septic tank
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2002-05-01
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References

  1. Caugant D. A., Levin B. R., Selander R. K. 1984; Distribution of multilocus genotypes of Escherichia coli within and between host families. J Hyg 92:377–384 [CrossRef]
     [Google Scholar]
  2. Cummings J. H., Bingham S. A., Heaton K. W., Eastwood M. A. 1992; Faecal weight, colon cancer risk, and dietary intake of nonstarch polysaccharides (dietary fibre). Gastroenterology 103:1783–1789
     [Google Scholar]
  3. Dombek P. E., Johnson L. K., Zimmerley S. T., Sadowsky M. J. 2000; Use of repetitive DNA sequences and the PCR to differentiate Escherichia coli isolates from human and animal sources. Appl Environ Microbiol 66:2572–2577 [CrossRef]
     [Google Scholar]
  4. Espinosa-Urgel M., Kolter R. 1998; Escherichia coli genes expressed preferentially in an aquatic environment. Mol Microbiol 28:325–332 [CrossRef]
     [Google Scholar]
  5. Excoffier L., Smouse P. E., Quattro J. M. 1992; Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491
     [Google Scholar]
  6. Gordon D. M. 2001; Geographical structure and host specificity in bacteria and the implications for tracing the source of coliform contamination. Microbiology 147:1079–1085
     [Google Scholar]
  7. Gordon D. M., Lee J. 1999; The genetic structure of enteric bacteria from Australian mammals. Microbiology 145:2673–2682
     [Google Scholar]
  8. Hartl D. L., Dykhuizen D. E. 1984; The population genetics of Escherichia coli . Annu Rev Genet 18:31–68 [CrossRef]
     [Google Scholar]
  9. Johnson J. R. 1991; Virulence factors in Escherichia coli . Clin Microbiol Rev 4:80–128
     [Google Scholar]
  10. Johnson J. R., Stell A. L. 2000; Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J Infect Dis 181:261–272 [CrossRef]
     [Google Scholar]
  11. Johnson J. R., Brown J. J., Carlino U. B., Russo T. A. 1998; Colonization with and acquisition of uropathogenic Escherichia coli as revealed by polymerase chain reaction-based detection. J Infect Dis 177:1120–1124 [CrossRef]
     [Google Scholar]
  12. Johnson J. R., Delavari P., Kuskowski M., Stell A. L. 2001a; Phylogenetic distribution of extraintestinal virulence-associated traits in Escherichia coli . J Infect Dis 183:78–88 [CrossRef]
     [Google Scholar]
  13. Johnson J. R., Stell A. L., Delavari P., Murray A. C., Kuskowski M., Gaastra W. 2001b; Phylogenetic and pathotypic similarities between Escherichia coli isolates from urinary tract infections in dogs and extraintestinal infections in humans. J Infect Dis 183:897–906 [CrossRef]
     [Google Scholar]
  14. Leclerc H., Mossel D. A. A., Edberg S. C., Struijk C. B. 2001; Advances in the bacteriology of the coliform group: their suitability as markers of microbial safety. Annu Rev Microbiol 55:201–234 [CrossRef]
     [Google Scholar]
  15. Nei M. 1978; Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590
     [Google Scholar]
  16. Okada S., Gordon D. M. 2001; Host and geographical factors influence the thermal niche of enteric bacteria isolated from native Australian mammals. Mol Ecol 10:2499–2513 [CrossRef]
     [Google Scholar]
  17. Parveen S., Murphree R. L., Edminston L., Kasper C. W., Portier K. M., Tamplin M. L. 1997; Association of multiple-antibiotic resistance profiles with point and nonpoint sources of Escherichia coli in Apalachicola Bay. Appl Environ Microbiol 63:2607–2612
     [Google Scholar]
  18. Parveen S., Portier K. M., Robinson K., Edmiston L., Tamplin M. L. 1999; Discriminant analysis of ribotype profiles of Escherichia coli for differentiating human and nonhuman sources of fecal pollution. Appl Environ Microbiol 65:3142–3147
     [Google Scholar]
  19. Power D. A., McCuen P. L. 1988 Manual of BBL Products and Laboratory Procedures , 6th edn. Cockeysville, MD: Becton Dickinson Microbial Systems;
     [Google Scholar]
  20. Pupo G. M., Richardson B. J. 1995; Biochemical genetics of a natural population of Escherichia coli : seasonal changes in alleles and haplotypes. Microbiology 141:1037–1044 [CrossRef]
     [Google Scholar]
  21. Savageau M. A. 1983; Escherichia coli habitats, cell types, and molecular mechanisms of gene control. Am Nat 122:732–744 [CrossRef]
     [Google Scholar]
  22. Siitonen A. 1994; What makes Escherichia coli pathogenic. Ann Med 4:229–231
     [Google Scholar]
  23. Whittam T. S. 1989; Clonal dynamics of Escherichia coli in its natural habitat. Antonie Leeuwenhoek 55:23–32 [CrossRef]
     [Google Scholar]
  24. Wright S. 1943; Isolation by distance. Genetics 28:114–138
     [Google Scholar]
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The genetic structure of Escherichia coli populations in primary and secondary habitats
Microbiology 148, 1513 (2002); https://doi.org/10.1099/00221287-148-5-1513
/content/journal/micro/10.1099/00221287-148-5-1513
/content/journal/micro/10.1099/00221287-148-5-1513
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