1887

Abstract

Differential fluorescence induction technology was used to identify promoters of genes that are expressed during lung infection of the mouse. Among the promoter clones that were identified multiple times was the promoter, which drives expression of the operon. These genes have been identified previously and shown to encode a manganese permease system as well as play a role in the virulence of this organism. Mutations in , or result in growth limitation in low manganese. The expression of the operon was examined and the virulence of deletion mutants of , , and was assessed in four different animal models of infection. The promoter was induced more than ten-fold using an intraperitoneal chamber implant model. The , and mutants were completely attenuated in systemic, respiratory tract and otitis media infections. In addition, these mutants were unable to grow in an implanted peritoneal chamber, but growth was restored by the addition of manganese to the chambers.

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2002-05-01
2019-10-17
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