1887

Abstract

Colonization of the human nasopharynx is a feature of some species of , and is a prerequisite of invasive meningococcal disease. The likelihood of colonization by varies widely between humans, and very few develop invasive disease. Explants of nasal mucosa derived from adult patients with non-allergic nasal obstruction were infected experimentally with spp. At intervals over 18 h incubation, washed explants were homogenized, and viable bacteria were counted. To estimate bacterial invasion of mucosa, explants were exposed to 025% sodium taurocholate for 30 s prior to homogenization. was recovered from the mucosa and the organism invaded and replicated within the tissue, in contrast to and (=9, <0008). isolates of clones ET-5, ET-37 and lineage III were recovered from and invaded tissue, but strains of clones A4, A:subgroup I, A:subgroup III and A:subgroup IV-1 did not invade (=6). To measure host variation, survival of within nasal mucosa of 40 different human donors was measured. Intra-class correlation of replicates was 097, but the coefficient of variation of recovered viable counts was 1335% after 4 h and 77% after 18 h incubation. It is concluded that the distinctive colonization and disease potential of spp. may be partly a consequence of their ability to invade and survive within human nasopharyngeal mucosa, but that this is influenced greatly by genetic or environmental factors operating on the host mucosa. This is consistent with the unpredictable epidemiology of meningococcal disease.

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2002-05-01
2020-01-20
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