%0 Journal Article %A Shaw, Robert K %A Daniell, Sarah %A Frankel, Gad %A Knutton, Stuart %T Enteropathogenic Escherichia coli translocate Tir and form an intimin–Tir intimate attachment to red blood cell membranes %D 2002 %J Microbiology, %V 148 %N 5 %P 1355-1365 %@ 1465-2080 %R https://doi.org/10.1099/00221287-148-5-1355 %K adhesion %K EPEC %K cell–cell interactions %K A/E, attaching and effacing %K DAPI, diamidino-2-phenylindole %K EPEC, enteropathogenic Escherichia coli %K RBC, red blood cell %K type III secretion %I Microbiology Society, %X Type III secretion allows bacteria to inject effector proteins into host cells. In enteropathogenic Escherichia coli (EPEC) the type III secreted protein, Tir, is translocated to the host-cell plasma membrane where it functions as a receptor for the bacterial adhesin intimin, leading to intimate bacterial attachment and ‘attaching and effacing’ (A/E) lesion formation. To study EPEC type III secretion the interaction of EPEC with monolayers of red blood cells (RBCs) has been exploited and in a recent study [Shaw, R. K., Daniell, S., Ebel, F., Frankel, G. & Knutton, S. (2001 R33 ). Cell Microbiol 3, 213–222] it was shown that EPEC induced haemolysis of RBCs and translocation of EspD, a putative pore-forming type III secreted protein in the RBC membrane. Here it is demonstrated that EPEC are able to translocate and correctly insert Tir into the RBC membrane and produce an intimin–Tir intimate bacterial attachment, identical to that seen in A/E lesions. Following translocation Tir did not undergo any change in apparent molecular mass or become tyrosine-phosphorylated and there was no focusing of RBC cytoskeletal actin beneath intimately adherent bacteria, and no pedestal formation. This study, employing an RBC model of infection, has demonstrated that Tir translocation can be separated from host-cell-mediated Tir modifications; the data show that the EPEC type III protein translocation apparatus is sufficient to deliver and correctly insert Tir into host-cell membranes independent of eukaryotic cell functions. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-5-1355