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Volume 148, Issue 5

Repression of expression reveals the functional importance of class IV chitin synthase gene in hyphal growth and conidiation of

The GenBank accession number for the the new version of the nucleotide sequence is D83246.

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Abstract

The functions of two previously identified chitin synthase genes in , and , were analysed. First, a conditional mutant was constructed in which the expression of is under the control of a repressible promoter, the promoter, of . Under repressing conditions, the mutant grew slowly and produced highly branched hyphae, supporting the idea that is involved in normal hyphal growth. The involvement of in conidiation was also demonstrated. Next, double mutants of and were constructed, in which was placed under the control of the promoter and was replaced with the gene of . These double mutants grew more slowly than the single mutant under high-osmolarity conditions. The hyphae of the double mutant appeared to be more disorganized than those of the single mutant. It was also found that ChsD was functionally implicated in conidiation when the expression of was limited. These results indicate the importance of the ChsD function in the absence of expression. The roles of ChsB and ChsD in hyphal growth and in conidiation were supported by the analysis of the spatial expression patterns of and , using of as a reporter gene.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): AP, alkaline phosphatase , cell wall , conidiation , filamentous fungi and RACE, rapid amplification of cDNA ends
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2002-05-01
2024-04-25
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Repression of chsB expression reveals the functional importance of class IV chitin synthase gene chsD in hyphal growth and conidiation of Aspergillus nidulansThe GenBank accession number for the the new version of the chsD nucleotide sequence is D83246.
Microbiology 148, 1335 (2002); https://doi.org/10.1099/00221287-148-5-1335
/content/journal/micro/10.1099/00221287-148-5-1335
/content/journal/micro/10.1099/00221287-148-5-1335
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