1887

Abstract

ABP-118, a small heat-stable bacteriocin produced by subsp. UCC118, a strain isolated from the ileal–caecal region of the human gastrointestinal tract, was purified to homogeneity. Using reverse genetics, a DNA fragment specifying part of ABP-118 was identified on a 10769 bp chromosomal region. Analysis of this region revealed that ABP-118 was a Class IIb two-peptide bacteriocin composed of Abp118α, which exhibited the antimicrobial activity, and Abp118β, which enhanced the antimicrobial activity. The gene conferring strain UCC118 immunity to the action of ABP-118, , was identified downstream of the β gene. Located further downstream of β, several ORFs were identified whose deduced proteins resembled those of proteins involved in bacteriocin regulation and secretion. Heterologous expression of ABP-118 was achieved in , and . In addition, the locus encoded an inducing peptide, AbpIP, which was shown to play a role in the regulation of ABP-118 production. This novel bacteriocin is, to the authors’ knowledge, the first to be isolated from a known human probiotic bacterium and to be characterized at the genetic level.

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2002-04-01
2019-10-15
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