1887

Abstract

The inability to transform many clinically important Gram-negative bacteria has hampered genetic studies addressing the mechanism of bacterial pathogenesis. This report describes the development and construction of a delivery system utilizing the broad-host-range transducing bacteriophage P1. The phagemids used in this system contain a P1 initiation site to package the vector, a P1 lytic replicon to generate concatemeric DNA, a broad-host-range origin of replication and an antibiotic-resistance determinant to select bacterial clones containing the recircularized phagemid. Phagemid DNA was successfully introduced by infection and stably maintained in members of the families (, , , and ) and (). In addition to laboratory strains, these virions were used successfully to deliver phagemids to a number of strains isolated from patients. This ability to deliver genetic information to wild-type strains raises the potential for use in antimicrobial therapies and DNA vaccine development.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-148-4-943
2002-04-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/148/4/1480943a.html?itemId=/content/journal/micro/10.1099/00221287-148-4-943&mimeType=html&fmt=ahah

References

  1. Antoine, R. & Locht, C. ( 1992; ). Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from Gram-positive organisms. Mol Microbiol 6, 1785-1799.[CrossRef]
    [Google Scholar]
  2. Arber, W. ( 1960; ). Transduction of chromosomal genes and episomes in Escherichia coli. Virology 11, 273-288.[CrossRef]
    [Google Scholar]
  3. Benedik, M. J. ( 1989; ). High efficiency transduction of single strand plasmid DNA into enteric bacteria. Mol Gen Genet 218, 353-354.[CrossRef]
    [Google Scholar]
  4. Bickle, T. A. & Krüger, D. H. ( 1993; ). Biology of DNA restriction. Microbiol Rev 57, 434-450.
    [Google Scholar]
  5. Chassy, B. M., Mercenier, A. & Flickinger, J. ( 1988; ). Transformation of bacteria by electroporation. Tibtech 6, 303-309.[CrossRef]
    [Google Scholar]
  6. Dinsmore, P. K. & Klaenhammer, T. R. ( 1995; ). Bacteriophage resistance in Lactococcus. Mol Biotechnol 4, 297-314.[CrossRef]
    [Google Scholar]
  7. Diver, J. M., Bryan, L. E. & Sokol, P. A. ( 1990; ). Transformation of Pseudomonas aeruginosa by electroporation. Anal Biochem 189, 75-79.[CrossRef]
    [Google Scholar]
  8. Dower, W. J., Miller, J. F. & Ragsdale, C. W. ( 1988; ). High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res 16, 6127-6145.[CrossRef]
    [Google Scholar]
  9. Elzer, P. H., Kovach, M. E., Phillips, R. W., Robertson, G. T., Peterson, K. M. & Roop, M. R. ( 1995; ). In vivo and in vitro stability of the broad-host-range cloning vector pBBR1MCS in six Brucella species. Plasmid 33, 51-57.[CrossRef]
    [Google Scholar]
  10. Gliesche, C. G. ( 1997; ). Transformation of methylotrophic bacteria by electroporation. Can J Microbiol 43, 197-201.[CrossRef]
    [Google Scholar]
  11. Glynn, M. K., Bopp, C., Dewitt, W., Dabney, P., Mokhtar, M. & Angulo, F. L. ( 1998; ). Emergence of multidrug-resistant Salmonella enterica serotype typhimurium DT104 infections in the United States. N Engl J Med 338, 1333-1338.[CrossRef]
    [Google Scholar]
  12. Gupta, K., Scholes, D. & Stamm, W. E. ( 1999; ). Increasing prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in women. JAMA (J Am Med Assoc) 281, 736-738.[CrossRef]
    [Google Scholar]
  13. Hanahan, D., Jessee, J. & Bloom, F. R. ( 1991; ). Plasmid transformation of Escherichia coli and other bacteria. Methods Enzymol 204, 63-113.
    [Google Scholar]
  14. Hancock, R. E. W. & Chapple, D. S. ( 1999; ). Peptide antibiotics. Antimicrob Agents Chemother 43, 1317-1323.
    [Google Scholar]
  15. Hochman, L., Segev, N., Sternberg, N. & Cohen, G. ( 1983; ). Site-specific recombinational circularization of bacteriophage P1 DNA. Virology 131, 11-17.[CrossRef]
    [Google Scholar]
  16. Iida, S., Streiff, M. B., Bickle, T. A. & Arber, W. ( 1987; ). Two DNA antirestriction systems of bacteriophage P1, darA, and darB: characterization of darA phages. Virology 157, 156-166.[CrossRef]
    [Google Scholar]
  17. Lawton, W. D. & Molnar, D. M. ( 1972; ). Lysogenic conversion of Pasteurella by Escherichia coli bacteriophage P1 Cm. J Virol 9, 708-709.
    [Google Scholar]
  18. Lennox, E. S. ( 1955; ). Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1, 190-206.[CrossRef]
    [Google Scholar]
  19. Lorenz, M. G. & Wackernagel, W. ( 1994; ). Bacterial gene transfer by natural genetic transformation in the environment. Microbiol Rev 58, 563-602.
    [Google Scholar]
  20. Miller, R. V. ( 1998; ). Bacterial gene swapping in nature. Sci Am 47, 67-71.
    [Google Scholar]
  21. Murooka, Y. & Harada, T. ( 1979; ). Expansion of the host range of coliphage P1 and gene transfer from enteric bacteria to other Gram-negative bacteria. Appl Environ Microbiol 30, 754-757.
    [Google Scholar]
  22. Novick, R. P. ( 1990; ). The staphylococcus as a molecular genetic system. In Molecular Biology of the Staphylococci , pp. 1-37. Edited by R. P. Novick. New York:VCH Publishers.
  23. Ogunseitan, O. A., Sayler, G. S. & Miller, R. V. ( 1992; ). Application of DNA probes to analysis of bacteriophage distribution patterns in the environment. Appl Environ Microbiol 58, 2046-2052.
    [Google Scholar]
  24. Okada, M. & Watanabe, T. ( 1968; ). Transduction with phage P1 in Salmonella typhimurium. Nature 218, 185-187.[CrossRef]
    [Google Scholar]
  25. Omenn, G. S. & Friedman, J. ( 1970; ). Isolation of mutants of Staphylococcus aureus lacking extracellular nuclease activity. J Bacteriol 101, 921-924.
    [Google Scholar]
  26. Pittet, D. & Wenzel, R. P. ( 1995; ). Nosocomial bloodstream infections. Secular trends in rates, mortality, and contribution to total hospital deaths. Arch Intern Med 155, 1177-1184.[CrossRef]
    [Google Scholar]
  27. Richard, P., Le Floch, R., Chamoux, C., Pannier, M., Espaze, E. & Richet, H. ( 1994; ). Pseudomonas aeruginosa outbreak in a burn unit: role of antimicrobials in the emergence of multiply resistant strains. J Infect Dis 170, 377-383.[CrossRef]
    [Google Scholar]
  28. Rosner, J. L. ( 1972; ). Formation, induction, and curing of bacteriophage P1 lysogens. Virology 49, 679-689.
    [Google Scholar]
  29. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  30. Segev, N. & Cohen, G. ( 1981; ). Control of circularization of bacteriophage P1 DNA in Escherichia coli. Virology 114, 333-342.[CrossRef]
    [Google Scholar]
  31. Shireen, T., Sarker, M. R. & Ahmed, Z. U. ( 1990; ). Studies on transformation in Shigella. Can J Microbiol 36, 348-351.[CrossRef]
    [Google Scholar]
  32. Snyder, L. ( 1995; ). Phage-exclusion enzymes: a bonanza of biochemical and cell biology reagents? Mol Microbiol 15, 415-420.[CrossRef]
    [Google Scholar]
  33. Sternberg, N. ( 1990; ). Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs. Proc Natl Acad Sci USA 87, 103-107.[CrossRef]
    [Google Scholar]
  34. Sternberg, N. & Hamilton, D. ( 1981; ). Bacteriophage P1 site-specific recombination. J Mol Biol 150, 467-486.[CrossRef]
    [Google Scholar]
  35. Sternberg, N., Sauer, B., Hoess, R. & Abremski, K. ( 1986; ). Bacteriophage P1 cre gene and its regulatory region. Evidence for multiple promoters and for regulation by DNA methylation. J Mol Biol 187, 197-212.[CrossRef]
    [Google Scholar]
  36. Su, H., Shao, Z., Tkalec, L., Blain, F. & Zimmerman, J. ( 2001; ). Development of a genetic system for the transfer of DNA into Flavobacterium heparinum. Microbiology 147, 581-589.
    [Google Scholar]
  37. Takagi, T. & Kisumi, M. ( 1985; ). Isolation of a versatile Serratia marcescens mutant as a host and molecular cloning of the aspartase gene. J Bacteriol 161, 1-6.
    [Google Scholar]
  38. Wiener, J., Quinn, J. P., Bradford, P. A., Goering, R. V., Nathan, C., Bush, K. & Weinstein, R. A. ( 1999; ). Multiple antibiotic-resistant Klebsiella and Escherichia coli in nursing homes. JAMA (J Am Med Assoc) 281, 517-523.[CrossRef]
    [Google Scholar]
  39. Wirth, R., Friesenegger, A. & Fiedler, S. ( 1989; ). Transformation of various species of Gram-negative bacteria belonging to 11 different genera by electroporation. Mol Gen Genet 216, 175-177.[CrossRef]
    [Google Scholar]
  40. Wright, J. J., Kumar, A. & Hayward, R. S. ( 1992; ). Hypersymmetry in a transcriptional terminator of Escherichia coli confers increased efficiency as well as bidirectionality. EMBO J 11, 1957-1964.
    [Google Scholar]
  41. Wu, S., Lo, S., Shao, C., Tsai, H. & Hor, L. ( 2001; ). Cloning and characterization of a periplasmic nuclease of Vibrio vulnificus and its role in preventing uptake of foreign DNA. Appl Environ Microbiol 67, 82-88.[CrossRef]
    [Google Scholar]
  42. Yarmolinsky, M. B. & Sternberg, N. ( 1988; ). Bacteriophage P1. In The Bacteriophages , pp. 291-438. Edited by R. Calendar. New York:Plenum.
  43. Zeph, L. R., Onaga, M. A. & Stotzky, G. ( 1988; ). Transduction of Escherichia coli by bacteriophage P1 in soil. Appl Environ Microbiol 54, 1731-1737.
    [Google Scholar]
  44. Zuercher, A. W., Miescher, S. M., Vogel, M., Rudolf, M. P., Stadler, M. B. & Stadler, B. M. ( 2000; ). Oral anti-IgE immunization with epitope-displaying phage. Eur J Immunol 30, 128-135.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-148-4-943
Loading
/content/journal/micro/10.1099/00221287-148-4-943
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error