1887

Abstract

Yeast plasma membrane Na/H antiporters (TC 2.A.36) share a high degree of similarity at the protein level. Expression of four antiporters ( Nha1p, Cnh1p, ZrSod2-22p and sod2p) in a mutant strain lacking both Na-ATPase and Na/H antiporter genes made it possible to study the transport properties and contribution to cell salt tolerance of all antiporters under the same conditions. The ZrSod2-22p of the osmotolerant yeast has the highest transport capacity for lithium and sodium but, like the sod2p, it does not recognize K and Rb as substrates. The Nha1p and Cnh1p have a broad substrate specificity for at least four alkali metal cations (Na, Li, K, Rb), but their contribution to overall cell tolerance to high external concentration of toxic Na and Li cations seems to be lower compared to the antiporters of and especially .

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2002-04-01
2019-10-21
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