1887

Abstract

produces an extracellular catalase termed M antigen, which is similar to catalase B of and species. Evidence is presented here for two additional catalase isozymes in . Catalase A is highly similar to a large-subunit catalase in and species, while catalase P is a small-subunit catalase protein with greatest similarity to known peroxisomal catalases of animals and yeasts. Complete cDNAs for the and genes (encoding catalases A and P, respectively) were isolated. The transcriptional expression of the , (M antigen) and genes was assessed by Northern blot hybridizations on total RNA. Results at the transcript levels for these genes are shown for three conditions: cell morphology (mycelial versus yeast phase cells), oxidative stress (in response to a challenge with HO) and carbon source (glucose vs glycerol). Collectively, these results demonstrated regulation of by both cell morphology and oxidative stress, but not by carbon source, and regulation of and by carbon source but not cell morphology or oxidative stress. A phylogenetic analysis of presently available catalase sequences and intron residences was done. The results support a model for evolution of eukaryotic monofunctional catalase genes from prokaryotic genes.

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2002-04-01
2019-09-19
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