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Volume 148, Issue 4

The Sup35p protein (CaSup35p): function, prion-like behaviour and an associated polyglutamine length polymorphism

The GenBank accession numbers for the sequences determined in this work are AF020554 ( gene) and AY028660–AY028673 ( N domains).

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Abstract

The Sup35p protein of is an essential translation factor whose prion-like properties give rise to the non-Mendelian genetic element [ ]. In this study the gene from the related yeast species has been characterized. The gene encodes a protein (CaSup35p) of 729 aa which shows 65% amino acid identity to the Sup35p protein (ScSup35p), with the C-terminal region showing greater identity (79%) than the N-terminal region. The full-length CaSup35p can functionally replace ScSup35p in although complementation is only complete when CaSup35p is overexpressed. Complementation only requires expression of the CaSup35p C domain. In the full-length CaSup35p is unable to establish a prion-like aggregated state even in the presence of endogenous ScSup35p prion ‘seeds’, thus confirming the existence of a species barrier in fungal prion propagation. Subcellular localization studies in show that although CaSup35p is normally ribosome-associated, when not ribosome-associated, it does not form pelletable high-molecular-mass aggregates characteristic of the ScSup35p in [ ] strains. Unlike the ScSup35p, the CaSup35p N domain contains a number of polyglutamine repeats although it does contain seven copies of the peptide GGYQQ that is repeated in the ScSup35p N domain. Analysis of the gene from 14 different strains of identified four naturally occurring polymorphisms associated with changes in the length of the largest of the polyglutamine repeats. These findings have important implications for the evolution of fungal prion genes.

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Keyword(s): [PSI+] determinant , polyQ , SUP35 gene and translation termination
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2002-04-01
2024-04-20
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The Candida albicans Sup35p protein (CaSup35p): function, prion-like behaviour and an associated polyglutamine length polymorphismThe GenBank accession numbers for the sequences determined in this work are AF020554 (CaSUP35 gene) and AY028660–AY028673 (CaSUP35 N domains).
Microbiology 148, 1049 (2002); https://doi.org/10.1099/00221287-148-4-1049
/content/journal/micro/10.1099/00221287-148-4-1049
/content/journal/micro/10.1099/00221287-148-4-1049
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