1887

Abstract

The 102 aa Hfq protein of (Hfq) was first described as a host factor required for phage Qβ replication. More recently, Hfq was shown to affect the stability of several mRNAs, including mRNA, where it interferes with ribosome binding, which in turn results in rapid degradation of the transcript. In contrast, Hfq is also required for efficient translation of the and gene, encoding the stationary σ factor. In this study, the authors have isolated and characterized the Hfq homologue of (Hfq), which consists of only 82 aa. The 68 N-terminal amino acids of Hfq show 92% identity with Hfq. Hfq was shown to functionally replace Hfq in terms of its requirement for phage Qβ replication and for expression. In addition, Hfq exerted the same negative effect on . mRNA expression. As judged by proteome analysis, the expression of either the plasmid-borne or the gene in an Hfq RpoS strain revealed no gross difference in the protein profile. Both Hfq and Hfq affected the synthesis of approximately 26 RpoS-independent gene products. These studies showed that the functional domain of Hfq resides within its N-terminal domain. The observation that a C-terminally truncated Hfq lacking the last 27 aa [Hfq] can also functionally replace the full-length protein lends further support to this notion.

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2002-03-01
2019-10-14
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