1887

Abstract

DivIVA appears to be a mediator of inhibition by MinCD of division at the cell poles in . Gel permeation and ultracentrifugation techniques were used to show self-association of DivIVA into a form consisting of 10–12 monomers . Western blot analysis of non-denaturating polyacrylamide gels confirms the presence of similar oligomers in cell lysates. These oligomers persist in a strain containing the mutation, in which proper vegetative septum positioning is abolished. In contrast, the mutation, which has a similar biological impact, appears to produce a protein with different oligomerization properties. The results of the present study suggest that oligomerization of DivIVA is important, but not sufficient for its function in the cell division process.

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2002-03-01
2020-01-22
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