1887

Abstract

Isocitrate lyase is the first enzyme of the glyoxylate shunt which is required for the assimilation of fatty acids and acetate. The intracellular pathogen contains high activities of this enzyme following growth on acetate and lactate, indicating that it plays an important role in the metabolism of these substrates. The gene encoding isocitrate lyase () was cloned and sequenced. It specifies a 46846 Da protein, which was shown to be functional by expressing it in . A gene similar to , encoding 3-hydroxyacyl-CoA dehydrogenase, was located 90 bp downstream from . Northern hybridization and RT-PCR experiments showed that and are cotranscribed into a 28 kb transcript. A smaller 16 kb transcript was also observed which was 25-fold more abundant than the - transcript. It is proposed that a stable hairpin structure with a free energy (Δ) of −285 kcal mol and located in the 90 bp - intergenic region is involved in stabilizing the transcript.

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2002-03-01
2020-04-07
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