1887

Abstract

is a primary colonizer of dental plaque and a major player in subacute bacterial endocarditis. In the present study, the authors report that an ORF (ORF3) located 77 bp downstream of the operon on the FW213 chromosome complements an thiol peroxidase () mutation in glutamine synthetase (GS) protection assays and that GS is protected by the ORF3 gene product in cell extracts. In addition, the putative streptococcal peroxidase (Tpx) protects from stress caused by HO and is induced by oxygen, as revealed by Northern blot analysis. Taken collectively, these findings support a thiol-dependent antioxidant activity for Tpx in .

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2002-03-01
2024-04-16
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References

  1. Britton L., Malinoswski D. P., Fridovich I. 1978; Superoxide dismutase and oxygen metabolism in Streptococcus faecalis and comparisons with other organisms. J Bacteriol 134:229–236
    [Google Scholar]
  2. Burnette-Curley D., Wells V., Viscount H., Munro C. L., Fenno J. C., Fives-Taylor P., Macrina F. L. 1995; FimA, a major virulence factor associated with Streptococcus parasanguis endocarditis. Infect Immun 63:4669–4674
    [Google Scholar]
  3. Cha M.-K., Kim H.-K., Kim I.-H. 1995; Thioredoxin-linked ‘thiol peroxidase’ from periplasmic space of Escherichia coli . J Biol Chem 270:28635–28641 [CrossRef]
    [Google Scholar]
  4. Cha M.-K., Kim H.-K., Kim I.-H. 1996; Mutation and mutagenesis of thiol peroxidase of Escherichia coli and a new type of thiol peroxidase family. J Bacteriol 178:5610–5614
    [Google Scholar]
  5. Chae H. Z., Uhm T. B., Rhee S. G. 1997; Dimerization of thiol-specific antioxidant and the essential role of cysteine 47. Proc Natl Acad Sci USA 91:7022–7026
    [Google Scholar]
  6. Dintilhac A., Claverys J.-P. 1997; The adc locus, which affects competence for genetic transformation in Streptococcus pneumoniae , encodes an ABC transporter with a putative lipoprotein homologous to a family of streptococcal adhesins. Res Microbiol 148:119–131 [CrossRef]
    [Google Scholar]
  7. Dintilhac A., Alloing G., Granadel C., Claverys J.-P. 1997; Competence and virulence of Streptococcus pneumoniae : Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases. Mol Microbiol 25:727–739 [CrossRef]
    [Google Scholar]
  8. Fenno J. C., Shaikh A., Fives-Taylor P. 1993; Characterization of allelic replacement in Streptococcus parasanguis: transformation and homologous recombination in a ‘non-transformable’ streptococcus. Gene 130:81–90 [CrossRef]
    [Google Scholar]
  9. Fenno J. C., Shaikh A., Spatafora G., Fives-Taylor P. 1995; The fimA locus of Streptococcus parasanguis encodes an ATP-binding membrane transport system. Mol Microbiol 15:849–863 [CrossRef]
    [Google Scholar]
  10. Fives-Taylor P. M., Thompson D. Q. 1985; Surface properties of Streptococcus sanguis FW213 mutants nonadherent to saliva-coated hydroxyapatite. Infect Immun 47:752–759
    [Google Scholar]
  11. Ganeshkumar N., Arora N., Kolenbrander P. 1993; Saliva-binding protein (SsaB) from Streptococcus sanguis 12 is a lipoprotein. J Bacteriol 175:572–574
    [Google Scholar]
  12. Hassett D. J., Sokol P. A., Howell M. L., Ma J. F., Schweizer H. T., Ochsner U., Vasil M. L. 1996; Ferric uptake regulator (Fur) mutants of Pseudomonas aeruginosa demonstrate defective siderophore-mediated iron uptake, altered aerobic growth, and decreased superoxide dismutase and catalase activities. J Bacteriol 178:3996–4003
    [Google Scholar]
  13. Jakubovics N. S., Smith A. W., Jenkinson H. F. 2000; Expression of the virulence-related Sca (Mn2+) permease in Streptococcus gordonii is regulated by a diphtheria toxin metallorepressor-like protein ScaR. Mol Microbiol 38:140–153 [CrossRef]
    [Google Scholar]
  14. Janulczyk R., Pallon J., Bjorck L. 1999; Identification and characterization of a Streptococcus pyogenes ABC transporter with multiple specificity for metal cations. Mol Microbiol 34:596–606 [CrossRef]
    [Google Scholar]
  15. Kim H.-K., Kim S.-J., Lee J.-W., Cha M.-K., Kim I.-H. 1996; Identification of a promoter in the 5′-flanking region of the E. coli thioredoxin-linked thiol peroxidase gene: evidence for the existence of oxygen-related transcriptional regulatory protein. Biochem Biophys Res Commun 221:641–646 [CrossRef]
    [Google Scholar]
  16. Kolenbrander P. E., Anderson R. N., Baker R. A., Jenkinson H. F. 1998; The adhesion-associated sca operon in Streptococcus gordonii encodes an inducible high-affinity ABC transporter for Mn2+ uptake. J Bacteriol 180:290–295
    [Google Scholar]
  17. Novak R., Braun J. S., Charpentier E., Tuomanan E. 1998; Penicillin tolerance genes in Streptococcus pneumoniae : the ABC-type manganese permease complex Psa. Mol Microbiol 29:1285–1296 [CrossRef]
    [Google Scholar]
  18. Oligino L., Fives-Taylor P. M. 1993; Overexpression and purification of a fimbria-associated adhesin of Streptococcus parasanguis . Infect Immun 61:1016–1022
    [Google Scholar]
  19. Rocha E. R., Selby T., Coleman J. P., Smith C. J. 1996; Oxidative stress response in an anaerobe, Bacteroides fragilis : a role for catalase in protection against hydrogen peroxide. J Bacteriol 178:6895–6903
    [Google Scholar]
  20. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Sampson J. S., O’Connor S. P., Stinson A. R., Tharpe J. A., Russell H. 1994; Cloning and nucleotide sequence analysis of psaA , the Streptococcus pneumoniae gene encoding a 37-kilodalton protein homologous to previously reported Streptococcus sp. adhesins. Infect Immun 62:319–324
    [Google Scholar]
  22. Spatafora G., Moore M., Landgren S., Stonehouse E., Michalek S. 2001; Expression of Streptococcus mutans fimA is iron-responsive and regulated by a DtxR homologue. Microbiology 147:1599–1610
    [Google Scholar]
  23. Spellerberg B., Rozdzinski E., Martin S., Wever-Heynemann J., Schnitzler N., Lutticken R., Podbielski A. 1999; Lmb, a protein with similarities to the LraI adhesion family, mediates attachment of Streptococcus agalactiae to human laminin. Infect Immun 67:871–878
    [Google Scholar]
  24. Stadtman E. R., Oliver C. N. 1991; Metal-catalyzed oxidation of proteins. J Biol Chem 266:2005–2008
    [Google Scholar]
  25. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354 [CrossRef]
    [Google Scholar]
  26. Wu H., Fives-Taylor P. 1999; Identification of dipeptide repeats and cell wall sorting signals in the fimbriae-associated adhesin, Fap1, of Streptococcus parasanguis . Mol Microbiol 34:1070–1081 [CrossRef]
    [Google Scholar]
  27. Zitzelsberger W., Gotz F., Schleifer K. H. 1984; Distribution of superoxide dismutases, oxidases and NADH peroxidase in various streptococci. FEMS Microbiol Lett 21:243–246 [CrossRef]
    [Google Scholar]
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