1887

Abstract

Chitinolytic bacteria are believed to be the primary aetiological agents of shell disease syndrome in marine crustaceans. The disease principally results from the breakdown of their chitinous exoskeletons by the shell disease pathogens, but pathogenicity may also manifest internally should a breach of the carapace occur. The current study looks at the pathogenicity of a number of bacteria (predominantly from the genus ) isolated from the edible crab, . All chitinase-producing bacteria investigated were capable of growth in a minimal medium consisting of chitin powder from crab shells, but differed in their speed of growth and nature of chitinolytic activity, suggesting that they have different roles within the lesion community. Two isolates (designated I4 and I7) were chosen for studies on internal pathogenicity, which included the effect of the pathogen on crab tissues, the ability of the host to remove the bacteria from circulation and the antibacterial activity of crab blood. Initially, I4 was rapidly removed from circulation, but began to reappear in the blood after 24 h. By 100 h, 100% of crabs were moribund. The septicaemic effects of the isolate were reflected in the low levels of its killing by blood-cell lysate and serum. By contrast, I7 was only slowly removed from circulation and caused the rapid mortality of all crabs in <3 h. A large decline in the number of circulating blood cells following injection of I7 was mirrored by an accumulation of these cells in the gills. Initial experiments suggest that the death of the crabs following injection with I7 may be caused by toxic extracellular bacterial products that exert their effects on the blood cells and nervous system of the crabs.

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2002-03-01
2019-12-07
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References

  1. Ayres, P. A. & Edwards, E. ( 1982; ). Notes on the distribution of ‘black spot’ shell disease in crustacean fisheries. Chem Ecol 1, 125-130.[CrossRef]
    [Google Scholar]
  2. Baross, J. A. & Tester, P. A. ( 1978; ). Incidence, microscopy, and etiology of exoskeletal lesions in the tanner crab, Chionoecetes tanneri. J Fish Res Board Can 35, 1141-1149.[CrossRef]
    [Google Scholar]
  3. Brimacombe, J. S. & Webber, J. M. (1964). Mucopolysaccharides. Amsterdam: Elsevier.
  4. Cerdà-Cuéllar, M., Jofre, J. & Blanch, A. R. ( 2000; ). A selective medium and a specific probe for detection of Vibrio vulnificus. Appl Environ Microbiol 66, 855-859.[CrossRef]
    [Google Scholar]
  5. Chisholm, J. R. S. & Smith, V. J. ( 1992; ). Antibacterial activity in the haemocytes of the shore crab, Carcinus maenas. J Mar Biol Assoc UK 72, 529-542.[CrossRef]
    [Google Scholar]
  6. Cipriani, G. R., Wheeler, R. S. & Sizemore, R. K. ( 1980; ). Characterization of brown spot disease of Gulf Coast shrimp. J Invertebr Pathol 36, 255-263.[CrossRef]
    [Google Scholar]
  7. dal Soglio, F. K., Bertagnolli, B. L., Sinclair, J. B., Yu, G. Y. & Eastburn, D. M. ( 1998; ). Production of chitinolytic enzymes and endoglucanase in the soybean rhizosphere in the presence of Trichoderma harzianum and Rhizoctonia solani. Biol Control 12, 111-117.[CrossRef]
    [Google Scholar]
  8. Delves-Broughton, J. & Poupard, C. W. ( 1976; ). Disease problems of prawns in recirculation systems in the UK. Aquaculture 7, 201-217.[CrossRef]
    [Google Scholar]
  9. Destoumieux, D., Bulet, P., Loew, D., Van Dorsselaer, A., Rodriguez, J. & Bachère, E. ( 1997; ). Penaeidins, a new family of antimicrobial peptides isolated from the shrimp Penaeus vannamei (Decapoda). J Biol Chem 272, 28398-28406.[CrossRef]
    [Google Scholar]
  10. Dietrich, M. A., Hackney, C. R. & Grodner, R. M. ( 1984; ). Factors affecting the adherence of Vibrio cholerae to blue crab (Callinectes sapidus) shell. In Vibrios in the Environment , pp. 601-611. Edited by R. R. Colwell. New York:Wiley.
  11. Dyrynda, E. A. ( 1998; ). Shell disease in the common shrimp Crangon crangon: variations within an enclosed estuarine system. Mar Biol 132, 445-452.[CrossRef]
    [Google Scholar]
  12. Frandberg, E. & Schnurer, J. ( 1994; ). Chitinolytic properties of Bacillus pabuli K1. J Appl Bacteriol 76, 361-367.[CrossRef]
    [Google Scholar]
  13. Getchell, R. G. ( 1989; ). Bacterial shell disease in crustaceans: a review. J Shellfish Res 8, 1-6.
    [Google Scholar]
  14. Høi, L. & Dalsgaard, A. ( 2000; ). Evaluation of a simplified semi-quantitative protocol for the estimation of Vibrio vulnificus in bathing water using cellobiose-colistin agar: a collaborative study with 13 municipal food controlling units in Denmark. J Microbiol Methods 41, 53-57.[CrossRef]
    [Google Scholar]
  15. Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T. & Williams, S. T. (1994). Bergey’s Manual of Determinative Bacteriology, 9th edn. Baltimore: Williams & Wilkins.
  16. Hood, M. A. & Meyers, S. P. ( 1977; ). Rates of chitin degradation in an estuarine environment. J Oceanogr Soc Jpn 33, 328-334.[CrossRef]
    [Google Scholar]
  17. Kang, S. C., Park, S. & Lee, D. G. ( 1999; ). Purification and characterization of a novel chitinase from the entomopathogenic fungus, Metarhizium anisopliae. J Invertebr Pathol 73, 276-281.[CrossRef]
    [Google Scholar]
  18. Keyhani, N. O. & Roseman, S. ( 1999; ). Physiological aspects of chitin catabolism in marine bacteria. Biochim Biophys Acta 1473, 108-122.[CrossRef]
    [Google Scholar]
  19. Liu, P. C., Lee, K. K., Tu, C. C. & Chen, S. N. ( 1997; ). Alkaline serine protease is an exotoxin of Vibrio alginolyticus in Kurama prawn, Penaeus japonicus. Curr Microbiol 34, 110-117.[CrossRef]
    [Google Scholar]
  20. McDougald, D., Rice, S. A., Weichart, D. & Kjelleberg, S. ( 1998; ). Nonculturability: adaptation or debilitation? FEMS Microbiol Ecol 25, 1-9.[CrossRef]
    [Google Scholar]
  21. Noga, E. J., Engel, D. P., Arroll, T. W., McKenna, S. & Davidian, M. ( 1994; ). Low serum antibacterial activity coincides with increased prevalence of shell disease in blue crabs Callinectes sapidus. Dis Aquat Org 19, 121-128.[CrossRef]
    [Google Scholar]
  22. Priest, F. G. ( 1977; ). Extracellular enzyme synthesis in the genus Bacillus. Bacteriol Rev 41, 711-753.
    [Google Scholar]
  23. Prince, D. L., Bayer, R. C., Gallagher, M. L. & Subramanyam, M. ( 1995; ). Reduction of shell disease with an experimental diet in a Nova-Scotian lobster pound. J Shellfish Res 14, 205-207.
    [Google Scholar]
  24. Roszak, D. B. & Colwell, R. R. ( 1987; ). Survival strategies of bacteria in the natural environment. Microbiol Rev 51, 365-379.
    [Google Scholar]
  25. Sawyer, T. K. ( 1991; ). Shell disease in the Atlantic rock crab, Cancer irroratus say, 1817, from the northeastern United States. J Shellfish Res 10, 495-497.
    [Google Scholar]
  26. Schlotfeldt, H.-J. ( 1972; ). Jahreszeitliche abhangigkeit der ‘schwarzfleckenkrankheit’ bei der garnele, Crangon crangon (L). Ber Wissen Komm Meeresfors 22, 397-399.
    [Google Scholar]
  27. Schnapp, D., Kemp, G. D. & Smith, V. J. ( 1996; ). Purification and characterization of a proline-rich antibacterial peptide, with sequence similarity to bactenecin-7, from the haemocytes of the shore crab, Carcinus maenas. Eur J Biochem 240, 532-539.[CrossRef]
    [Google Scholar]
  28. Smith, V. J. & Ratcliffe, N. A. ( 1980; ). Host defence reactions in the shore crab, Carcinus maenus (L.): clearance and distribution of test particles. J Mar Biol Assoc UK 60, 89-102.[CrossRef]
    [Google Scholar]
  29. Smith, V. J. & Ratcliffe, N. A. ( 1981; ). Pathological changes in the nephrocytes of the shore crab, Carcinus maenas, following injection of bacteria. J Invertebr Pathol 38, 113-121.[CrossRef]
    [Google Scholar]
  30. Smolowitz, R. M., Bullis, R. A. & Abt, D. A. ( 1992; ). Pathological cuticular changes of winter impoundment shell disease preceding and during intermolt in the American lobster, Homarus americanus. Biol Bull 183, 99-112.[CrossRef]
    [Google Scholar]
  31. Stewart, J. E. ( 1993; ). Infectious diseases of marine crustaceans. In Pathobiology of Marine and Estuarine Organisms , pp. 319-342. Edited by J. A. Couch & J. W. Fournie. Boca Raton:CRC Press.
  32. Sudheesh, P. S. & Xu, H. S. ( 2001; ). Pathogenicity of Vibrio parahaemolyticus in tiger prawn Penaeus monodon fabricius: possible role of extracellular proteases. Aquaculture 196, 37-46.[CrossRef]
    [Google Scholar]
  33. Tronsmo, A. & Harman, G. E. ( 1993; ). Detection and quantification of N-acetyl-β-d-glucosaminidase, chitobiosidase, and endochitinase in solutions and on gels. Anal Biochem 208, 74-79.[CrossRef]
    [Google Scholar]
  34. Urakawa, H., Kita-Tsukamoto, K. & Ohwada, K. ( 1999; ). Restriction fragment length polymorphism analysis of psychrophilic and psychrotrophic Vibrio and Photobacterium from the north-western Pacific Ocean and Otsuchi Bay, Japan. Can J Microbiol 45, 67-76.[CrossRef]
    [Google Scholar]
  35. Vogan, C. L. & Rowley, A. F. (2002). Effects of shell disease syndrome on the haemocytes and humoral defences of the edible crab, Cancer pagurus. Aquaculture (in press).
  36. Vogan, C. L., Llewellyn, P. J. & Rowley, A. F. ( 1999; ). Epidemiology and dynamics of shell disease in the edible crab Cancer pagurus: a preliminary study of Langland Bay, Swansea, UK. Dis Aquat Org 35, 81-87.[CrossRef]
    [Google Scholar]
  37. Vogan, C. L., Costa-Ramos, C. & Rowley, A. F. ( 2001; ). A histological study of shell disease syndrome in the edible crab, Cancer pagurus. Dis Aquat Org 47, 209-217.[CrossRef]
    [Google Scholar]
  38. Wardell, J. N. ( 1988; ). Methods for the study of bacterial attachment. In Methods in Aquatic Bacteriology , pp. 389-415. Edited by B. Austin. Chichester:Wiley.
  39. Yanai, K., Takaya, N., Kojima, N., Horiuchi, H., Ohta, A. & Takagi, M. ( 1992; ). Purification of 2 chitinases from Rhizopus oligosporus and isolation and sequencing of the encoding genes. J Bacteriol 174, 7398-7406.
    [Google Scholar]
  40. Young, J. S. ( 1991; ). Prevalence and severity of shell disease among deep-sea red crabs (Chaceon quinquedens, Smith 1879) in relation to ocean dumping of sewage sludge. J Shellfish Res 10, 499-503.
    [Google Scholar]
  41. Young, J. S. & Pearce, J. B. ( 1975; ). Shell disease from crabs and lobsters from New York Bight. Mar Pollut Bull 6, 101-105.
    [Google Scholar]
  42. Zhang, X. H. & Austin, B. ( 2000; ). Pathogenicity of Vibrio harveyi to salmonids. J Fish Dis 23, 93-102.[CrossRef]
    [Google Scholar]
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