1887

Abstract

Bile-tolerant spp. are emerging human and animal pathogens. However, due to their fastidious nature, which requires nutrient-rich complex media to grow, infection with these bacteria may be underestimated. The accumulation of toxic metabolites in cultures may be one of the main obstacles for successful culture of these organisms. The present study examined various potential growth-enhancing substances for spp. and, furthermore, how they may affect spiral to coccoid conversion. Five spp. were cultured on agar and in broth media supplemented with activated charcoal, β-cyclodextrin, or porcine gastric mucin. Growth was determined by estimating the numbers of colony-forming units and colony diameter, as well as bacterial cell mass. Coccoid transformation was estimated every 24 h by both Gram and acridine-orange staining. Activated charcoal was superior in supporting growth and increased cell mass on agar and in broth media. β-Cyclodextrin delayed spiral to coccoid conversion by and , whereas activated charcoal delayed the conversion to coccoid forms of and . The progression to coccoid forms by on agar media was not influenced by any growth supplement. The spiral to coccoid conversion was more rapid in broth media than on agar media. The growth enhancement observed is probably related to the capacity of activated charcoal to remove toxic compounds in culture media.

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2002-03-01
2019-10-15
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References

  1. Barer, M. R., Gribbon, L. T., Harwood, C. R. & Nwoguh, C. E. ( 1993; ). The viable but non-culturable hypothesis and medical bacteriology. Rev Med Microbiol 4, 183-191.[CrossRef]
    [Google Scholar]
  2. Benaissa, M., Babin, P., Quellard, N., Pezennec, L., Cenatiempo, Y. & Faucher, J. L. ( 1996; ). Changes in Helicobacter pylori ultrastructure and antigens during conversion from the bacillary to coccoid form. Infect Immun 64, 2331-2335.
    [Google Scholar]
  3. Bradshaw, D. J., Homer, K. A., Marsh, P. D. & Beighton, D. ( 1994; ). Metabolic cooperation in oral microbial communities growth on mucin. Microbiology 140, 3407-3412.[CrossRef]
    [Google Scholar]
  4. Buck, G. E. & Smith, J. S. ( 1987; ). Medium supplementation for growth of Campylobacter pyloridis. J Clin Microbiol 25, 597-599.
    [Google Scholar]
  5. Cellini, L., Allocati, N., Di-Campli, E. & Dainelli, B. ( 1994; ). Helicobacter pylori: a fickle germ. Microbiol Immunol 38, 25-30.[CrossRef]
    [Google Scholar]
  6. Chan, W. Y., Hui, P. K., Leung, K. M., Chow, J., Kwok, F. & Ng, C.-S. ( 1994; ). Coccoid forms of Helicobacter pylori in the human stomach. Am J Pathol 102, 503-507.
    [Google Scholar]
  7. Costa, K., Bacher, G., Allmaier, G., Dominguez-Bello, M. G., Engstrand, L., Falk, P., de Pedro, M. A. & Garcia-del Portillo, F. ( 1999; ). The morphological transition of Helicobacter pylori cells from spiral to coccoid is preceded by a substantial modification of the cell wall. J Bacteriol 181, 3710-3715.
    [Google Scholar]
  8. Coudron, P. & Statton, C. W. ( 1995; ). Factors effect growth and susceptibility testing of Helicobacter pylori in liquid media. J Clin Microbiol 33, 1028-1030.
    [Google Scholar]
  9. Donelli, G., Matarrese, P., Fiorentini, C., Dainelli, B., Taraborelli, T., Di Campli, E., Di Bartolomeo, S. & Cellini, L. ( 1998; ). The effect of oxygen on the growth and cell morphology of Helicobacter pylori. FEMS Microbiol Lett 168, 9-15.[CrossRef]
    [Google Scholar]
  10. Eaton, K. A., Dewhirst, F. E., Radin, M. J., Fox, J. G., Paster, B. J., Krakowka, S. & Morgan, D. R. ( 1993; ). Helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. Int J Syst Microbiol 43, 99-106.
    [Google Scholar]
  11. Edelstein, P. H. & Edelstein, M. A. C. ( 1993; ). Comparison of three buffers used in the formation of buffer charcoal yeast extract medium. J Clin Microbiol 31, 3329-3330.
    [Google Scholar]
  12. Ermolaeva, S., Belyi, Y. & Tartakovskii, I. ( 1999; ). Characteristics of induction of virulence factor expression by activated charcoal in Listeria monocytogenes. FEMS Microbiol Lett 174, 137-141.[CrossRef]
    [Google Scholar]
  13. Forman, D. ( 1996; ). Helicobacter pylori and gastric cancer. Scand J Gastroenterol 251, 48-51.
    [Google Scholar]
  14. Fox, J. G., Yan, L., Dewhirst, F. E., Paster, B. J., Shames, B., Murphy, J. C., Hayward, A., Belcher, J. C. & Mendes, E. N. ( 1995; ). Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, liver, and intestine of aged inbred mice. J Clin Microbiol 33, 445-454.
    [Google Scholar]
  15. Fox, J. G., Drolet, R., Higgins, R., Messier, S., Yan, L., Coleman, B. E., Paster, P. J. & Dewhirst, F. E. ( 1996; ). Helicobacter canis isolated from dog liver with multifocal necrotizing hepatitis. J Clin Microbiol 34, 2479-2482.
    [Google Scholar]
  16. Fox, J. G., Dewhirst, F. E., Shen, Z. & 8 other authors ( 1998; ). Hepatic Helicobacter species identified in bile and gallbladder tissues in Chileans with chronic cholecystitis. Gastroenterology 114, 755–767.[CrossRef]
    [Google Scholar]
  17. Franklin, C. L., Beckwith, C. S., Livingston, R. S., Riley, L. K., Gibson, S. V., Besch-Williford, C. V. & Hook, R. R. ( 1996; ). Isolation of a novel Helicobacter cholecystus sp. from the gallbladder of Syrian hamsters with cholangiofibrosis and centrilobular pancreatitis. J Clin Microbiol 4, 2952-2958.
    [Google Scholar]
  18. Goodwin, C. S., Mendall, M. M. & Northfield, T. C. ( 1997; ). Helicobacter pylori infection. Lancet 349, 265-269.[CrossRef]
    [Google Scholar]
  19. Hazell, S. L., Markesich, D. C., Evans, D. J. & Graham, D. Y. ( 1989; ). Influence of media supplements on growth and survival of Campylobacter pylori. Eur J Microbiol 8, 597-602.[CrossRef]
    [Google Scholar]
  20. Hoppe, J. E. & Schwaderer, J. ( 1989; ). Comparison of four media for the isolation of Bordetella pertussis. J Clin Microbiol 27, 1097-1098.
    [Google Scholar]
  21. Issam, R., Rand, K. & Gaskins, D. ( 1990; ). Buffered charcoal-yeast extract medium for the isolation of Brucella. J Clin Microbiol 28, 1671-1672.
    [Google Scholar]
  22. Jalava, K., Kartinen, M., Utriainen, M., Haponen, I. & Hänninen, M. I. ( 1997; ). Helicobacter salomonis sp. nov., a canine gastric Helicobacter sp. related to Helicobacter felis and Helicobacter bizzozeronii. Int J Syst Bacteriol 47, 975-982.[CrossRef]
    [Google Scholar]
  23. Kusters, J. G., Gerrits, M. M., Van Strijp, J. A. G. & Vandenbroucke-Grauls, C. M. J. E. ( 1997; ). Coccoid forms of Helicobacter pylori are the morphologic manifestation of cell death. Infect Immun 65, 3672-3679.
    [Google Scholar]
  24. Lee, A., Phillips, M. W., O’Rourke, J. L. & 7 other authors ( 1992; ). Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from intestinal mucosa of rodents. Int J Syst Bacteriol 42, 27–36.[CrossRef]
    [Google Scholar]
  25. Marchini, A., d’Apolito, M., Massari, P., Atzeni, M., Copass, M. & Olivieri, R. ( 1995; ). Cyclodextrins for growth of Helicobacter pylori and production of vacuolating cytotoxin. Arch Microbiol 164, 290-293.[CrossRef]
    [Google Scholar]
  26. Moran, A. P. ( 1997; ). Coccoid forms of Helicobacter pylori. Helicobacter 2, 109-110.
    [Google Scholar]
  27. Moran, A. P. & Upton, M. E. ( 1986; ). A comparative study of the rod and coccoid forms of Campylobacter jejuni ATCC 29428. J Appl Bacteriol 60, 103-110.[CrossRef]
    [Google Scholar]
  28. Moran, A. P. & Upton, M. E. ( 1987a; ). Effect of medium supplements, illumination and superoxide dismutase on the production of coccoid forms of Campylobacter jejuni ATCC 29428. J Appl Bacteriol 62, 43-51.[CrossRef]
    [Google Scholar]
  29. Moran, A. P. & Upton, M. E. ( 1987b; ). Factors affecting production of coccoid forms by Campylobacter jejuni on solid media during incubation. J Appl Bacteriol 62, 527-537.[CrossRef]
    [Google Scholar]
  30. Narikawa, S., Kawai, S., Aoshima, H., Kawamata, O., Kawaguchi, R., Hikiji, K., Kato, M., Iino, S. & Mizushima, Y. ( 1997; ). Comparison of the nucleic acids of helical and coccoid forms of Helicobacter pylori. Clin Diagn Lab Immunol 4, 285-290.
    [Google Scholar]
  31. Nilsson, H.-O., Taneera, J., Castedal, M., Glatz, E., Olsson, R. & Wadström, T. ( 2000a; ). Identification of Helicobacter pylori and other Helicobacter species by PCR, hybridization and partial DNA sequencing in human liver samples from patients with primary sclerosing cholangitis or primer biliary cirrhosis. J Clin Microbiol 38, 1072-1076.
    [Google Scholar]
  32. Nilsson, I., Lindgren, S., Eriksson, S. & Wadström, T. ( 2000b; ). Serum antibodies to Helicobacter hepaticus and Helicobacter pylori in patients with liver disease. Gut 46, 410-414.[CrossRef]
    [Google Scholar]
  33. Norris, C. R., Marks, S. L., Eaton, K. A., Torabian, S. Z., Munn, R. J. & Solnick, J. V. ( 1999; ). Healthy cats are commonly colonized with Helicobacter heilmannii that is associated with minimal gastritis. J Clin Microbiol 37, 189-194.
    [Google Scholar]
  34. Roberton, A. M. & Stanley, R. A. ( 1982; ). In vitro utilization of mucin by Bacteroides fragilis. Appl Environ Microbiol 43, 325-330.
    [Google Scholar]
  35. Shahamat, M., Mai, H. E. U., Paszko-Kolva, C., Yamamoto, H. & Colwell, R. R. ( 1991; ). Evaluation of liquid media for growth of Helicobacter pylori. J Clin Microbiol 29, 2835-2837.
    [Google Scholar]
  36. Signoretto, C., del Mar Lleo, M., Tafi, M. C. & Canepari, P. ( 2000; ). Cell wall chemical composition of Enterococcus faecalis in the viable but non-culturable state. Appl Environ Microbiol 66, 1953-1959.[CrossRef]
    [Google Scholar]
  37. Soltesz, V. L. & Mårdh, P. ( 1980; ). Serum free liquid media for Neisseria gonorrhoeae. Curr Microbiol 4, 45-49.[CrossRef]
    [Google Scholar]
  38. Soltesz, V., Zeeberg, B. & Wadström, T. ( 1992; ). Optimal survival of Helicobacter pylori under various transport conditions. J Clin Microbiol 30, 1453-1456.
    [Google Scholar]
  39. Stanley, J., Linton, D., Burnens, A. P., Dewhirst, F. E., On, S. L., Porter, A., Owen, R. J. & Costas, M. ( 1994; ). Helicobacter pullorum sp. nov., genotype and phenotype of a new species isolated from poultry and from humans with gastroenteritis. Microbiology 140, 3441-3449.[CrossRef]
    [Google Scholar]
  40. Versalovic, J. & Fox, J. G. ( 2001; ). Taxonomy and phylogeny of Helicobacter. In Helicobacter pylori: Molecular and Cellular Biology , pp. 15-28. Edited by M. Achtman & S. Suerbaum. Wymondham, Norfolk, England:Horizon Scientific Press.
  41. Walsh, E. J. & Moran, A. P. ( 1997; ). Influence of medium composition on the growth and antigen expression of Helicobacter pylori. J Appl Microbiol 83, 67-75.[CrossRef]
    [Google Scholar]
  42. Ward, J. M., Fox, J. G., Anver, M. B., Haines, D. C., George, C. V. & Collins, M. L. ( 1994; ). Chronic active hepatitis and associated liver tumours in mice caused by persistent bacterial infection with a novel Helicobacter species. J Natl Cancer Inst 86, 1222-1227.[CrossRef]
    [Google Scholar]
  43. West, A. P., Millar, M. R. & Tompkins, D. S. ( 1990; ). Survival of Helicobacter pylori in water and saline. J Clin Pathol 43, 409.
    [Google Scholar]
  44. Worku, M. L., Sidebotham, R. L., Walker, M. M., Keshavarz, T. & Karim, Q. N. ( 1999; ). The relationship between Helicobacter pylori motility, morphology and phase of growth: implications for gastric colonization and pathology. Microbiology 145, 2803-2811.
    [Google Scholar]
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