Mutagenesis of conserved tryptophan residues within the receptor-binding domain of intimin: influence on binding activity and virulence

References

1. Abe A., Pfuetzner R. A., Sanchez-Sanmartin C., Devinney R., Puente J. L., Strynadka N. C., Finlay B. B., de Grado M. 1999; Enteropathogenic Escherichia coli translocated intimin receptor, Tir, requires a specific chaperone for stable secretion. Mol Microbiol 33:1162–1175
   [Google Scholar]
2. Adu-Bobie J., Frankel G., Bain C., Goncaleves A. G., Trabulsi L. R., Douce G., Knutton S., Dougan G. 1998; Detection of intimin α, β, γ, and δ, four intimin derivatives expressed by attaching and effacing microbial pathogens. J Clin Microbiol 36:662–668
   [Google Scholar]
3. Barthold S. W., Coleman G. L., Jacoby R. O., Livstone E. M., Jonas A. M. 1976; Transmissiable murine colonic hyperplasia. Vet Pathol 15:223–236
   [Google Scholar]
4. Batchelor M., Prasannan S., Daniell S., Reece S., Connerton I., Bloomberg G., Dougan G., Frankel G., Matthews S. 2000; Structural basis for recognition of the translocated intimin receptor (Tir) by intimin from enteropathogenic Escherichia coli . EMBO J 19:2452–2464 [CrossRef]
   [Google Scholar]
5. Dean-Nystrom E. A., Bosworth B. T., Moon H. W., O’Brien A. D. 1998; Escherichia coli O157: H7 requires intimin for enteropathogenicity in calves. Infect Immun 66:4560–4563
   [Google Scholar]
6. Deibel C., Kramer S., Chakraborty T., Ebel F. 1998; EspE, a novel secreted protein of attaching and effacing bacteria, is directly translocated into infected host cells, where it appears as a tyrosine-phosphorylated 90 kDa protein. Mol Microbiol 28:463–474 [CrossRef]
   [Google Scholar]
7. Donnenberg M. S., Kaper J. B. 1991; Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect Immun 59:4310–4317
   [Google Scholar]
8. Donnenberg M. S., Tacket C. O., James S. P., Losonsky G., Nataro J. P., Wasserman S. S., Kaper J. B., Levine M. M. 1993a; Role of the eaeA gene in experimental enteropathogenic Escherichia coli infection. J Clin Invest 92:1412–1417 [CrossRef]
   [Google Scholar]
9. Donnenberg M. S., Tzipori S., McKee M. L., O’Brien A. D., Alroy J., Kaper J. B. 1993b; The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model. J Clin Invest 92:1418–1424 [CrossRef]
   [Google Scholar]
10. Elliott S. J., Wainwright L. A., McDaniel T. K., Jarvis K. G., Deng Y. K., Lai L. C., McNamara B. P., Donnenberg M. S., Kaper J. B. 1998; The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol Microbiol 28:1–4
    [Google Scholar]
11. Elliott S. J., Dubois M. S., Hutcheson S. W., Wainwright L. A., Batchelor M., Frankel G., Knutton S., Kaper J. B. 1999; Identification of CesT, a chaperone for the type III secretion of Tir in Enteropathogenic Escherichia coli . Mol Microbiol 33:1176–1189
    [Google Scholar]
12. Frankel G., Candy D. C., Everest P., Dougan G. 1994; Characterization of the C-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic Escherichia coli , Citrobacter freundii , and Hafnia alvei . Infect Immun 62:1835–1842
    [Google Scholar]
13. Frankel G., Candy D. C., Fabiani E., Adu-Bobie J., Gil S., Novakova M., Phillips A. D., Dougan G. 1995; Molecular characterization of a carboxy-terminal eukaryotic-cell-binding domain of intimin from enteropathogenic Escherichia coli . Infect Immun 63:4323–4328
    [Google Scholar]
14. Frankel G., Lider O., Hershkoviz R., Mould A. P., Kachalsky S. G., Candy D. C. A., Cahalon L., Humphries M. J., Dougan G. 1996; The cell-binding domain of intimin from enteropathogenic Escherichia coli binds to β1 integrins. J Biol Chem 271:20359–20364 [CrossRef]
    [Google Scholar]
15. Frankel G., Philips A. D., Novakova M., Batchelor M., Hicks S., Dougan G. 1998a; Generation of Escherichia coli intimin-derivatives with differing biological activities using site-directed mutagenesis of the intimin C terminus domain. Mol Microbiol 29:559–570 [CrossRef]
    [Google Scholar]
16. Frankel G., Phillips A. D., Rosenshine I., Dougan G., Kaper J. B., Knutton S. 1998b; Enteropathogenic and enterohaemorrhagic Escherichia coli : more subversive elements. Mol Microbiol 30:911–921 [CrossRef]
    [Google Scholar]
17. Ghaem-Maghami M., Simmons C. P., Daniell S., Pizza M., Lewis D., Frankel G., Dougan G. 2001; Intimin-specific immune responses prevent bacterial colonization by the attaching–effacing pathogen Citrobacter rodentium . Infect Immun 69:5597–5605 [CrossRef]
    [Google Scholar]
18. Hartland E. L., Batchelor M., Delahay R. M., Hale C., Matthews S., Dougan G., Knutton S., Connerton I., Frankel G. 1999; Binding of intimin from enteropathogenic Escherichia coli to Tir and to host cells. Mol Microbiol 32:151–158 [CrossRef]
    [Google Scholar]
19. Hartland E. L., Huter V., Higgins L. M., Goncalves N. S., Dougan G., Phillips A. D., MacDonald T. T., Frankel G. 2000; Expression of intimin gamma from enterohemorrhagic Escherichia coli in Citrobacter rodentium . Infect Immun 68:4637–4646 [CrossRef]
    [Google Scholar]
20. Higgins L. M., Frankel G., Connerton I., Goncalves N. S., Dougan G., MacDonald T. T. 1999a; Role of bacterial intimin in colonic hyperplasia and inflammation. Science 285:588–591 [CrossRef]
    [Google Scholar]
21. Higgins L. M., Frankel G., Douce G., Dougan G., MacDonald T. T. 1999b; Citrobacter rodentium infection in mice elicits a mucosal Th1 cytokine response and lesions similar to those in murine inflammatory bowel disease. Infect Immun 67:3031–3139
    [Google Scholar]
22. Jerse A. E., Kaper J. B. 1991; The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid. Infect Immun 59:4302–4309
    [Google Scholar]
23. Jerse A. E., Yu J., Tall B. D., Kaper J. B. 1990; A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci USA 87:7839–7843 [CrossRef]
    [Google Scholar]
24. Kalman D., Weiner O. D., Goosney D. L., Sedat J. W., Finlay B. B., Abo A., Bishop J. M. 1999; Enteropathogenic E. coli acts through WASP and Arp2/3 complex to form actin pedestals. Nat Cell Biol 1:389–391 [CrossRef]
    [Google Scholar]
25. Kaper J. B., Elliot S., Sperandio V., Perna T. P., Maythew G. F., Blatner F. R. 1998; Attaching-and-effacing intestinal histopathology and locus of enterocyte effacement. In Escherichia coli and Other Shiga Toxin-Producing E. coli Strains pp 163–182 Edited by Kaper J. B. O’Brien A. D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
26. Kenny B., DeVinney R., Stein M., Reinscheid D. J., Frey E. A., Finlay B. B. 1997; Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell 91:511–520 [CrossRef]
    [Google Scholar]
27. Knutton S., Baldwin T., Williams P. H., McNeish A. S. 1989; Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli . Infect Immun 57:1290–1298
    [Google Scholar]
28. Levine M. M., Nataro J. P., Karch H., Baldini M. M., Kaper J. B., Black R. E., Clements M. L., O’Brien A. D. 1985; The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor. J Infect Dis 152:550–559 [CrossRef]
    [Google Scholar]
29. Luo Y., Frey E. A., Pfuetzner R. A., Creagh A. L., Knoechel D. G., Haynes C. A., Finlay B. B., Strynadka N. C. 2000; Crystal structure of enteropathogenic Escherichia coli intimin-receptor complex. Nature 405:1073–1077 [CrossRef]
    [Google Scholar]
30. Marches O., Nougayrede J. P., Boullier S. 8 other authors 2000; Role of tir and intimin in the virulence of rabbit enteropathogenic Escherichia coli serotype O103: H2. Infect Immun 68:2171–2182 [CrossRef]
    [Google Scholar]
31. McDaniel T. K., Jarvis K. G., Donnenberg M. S., Kaper J. B. 1995; A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc Natl Acad Sci USA 92:1664–1668 [CrossRef]
    [Google Scholar]
32. McKee M. L., Melton-Celsa A. R., Moxley R. A., Francis D. H., O’Brien A. D. 1995; Enterohemorrhagic Escherichia coli O157: H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells. Infect Immun 63:3739–3744
    [Google Scholar]
33. Mellies J. L., Elliott S. J., Sperandio V., Donnenberg M. S., Kaper J. B. 1999; The Per regulon of enteropathogenic Escherichia coli : identification of a regulatory cascade and a novel transcriptional activator, the locus of enterocyte effacement (LEE)-encoded regulator (Ler). Mol Microbiol 33:296–306 [CrossRef]
    [Google Scholar]
34. Nataro J. P., Kaper J. B. 1998; Diarrheagenic Escherichia coli . Clin Microbiol Rev 11:142–201
    [Google Scholar]
35. Oswald E., Schmidt H., Morabito S., Karch H., Marches O., Caprioli A. 2000; Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. Infect Immun 68:64–71 [CrossRef]
    [Google Scholar]
36. Reece S., Simmons C., Fitzhenry R. J., Matthews S., Phillips A. D., Dougan G., Frankel G. 2001; Site directed mutagenesis of intimin α modulates intimin-mediated tissue tropism and host specificity. Mol Microbiol 40:86–98 [CrossRef]
    [Google Scholar]
37. Schauer D. B., Falkow S. 1993a; Attaching and effacing locus of a Citrobacter freundii biotype 4280 that causes transmissible murine colonic hyperplasia. Infect Immun 61:2486–2492
    [Google Scholar]
38. Schauer D. B., Falkow S. 1993b; The eae gene of Citrobacter freundii biotype 4280 is necessary for colonization in transmissible murine colonic hyperplasia. Infect Immun 61:4654–4661
    [Google Scholar]
39. Weis W. I., Drickamer K. 1996; Structural basis of lectin–carbohydrate recognition. Annu Rev Biochem 65:441–473 [CrossRef]
    [Google Scholar]