1887

Microbiology

Volume 148, Issue 3

Intragenomic and intraspecific polymorphism of the 16S–23S rDNA internally transcribed sequences of

The GenBank accession numbers for the sequences reported in this paper can be found in Table 1.

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Abstract

The nucleotide composition of the internally transcribed sequences (ITSs) of the six rDNA operons of two strains of were determined. Four variable and five conserved nucleotide blocks were distinguished. Five different modular organizations were revealed for each strain and no homologous loci showed the same succession of blocks. This suggests that recombination frequently occurs between the rDNA loci, leading to the exchange of nucleotide blocks. The modular structure was also observed within the ITSs of M145, which is closely related to , and 2247, showing the same number of constant blocks but with fewer variable regions. This confirms that a high degree of ITS variability is a common characteristic among spp. The functional significance of the combinations of variable and constant nucleotide blocks of the ITS was examined by prediction of secondary structures from nucleotide sequences. The secondary structures were shown to be analogous whatever the combination of variable/constant blocks at the intragenomic, intraspecific and interspecific levels.

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ATCC, American Type Culture Collection , c, constant region , DSM, Deutsche Sammlung von Mikroorganismen und Zelkulturen , dsPS, double-stranded processing site , gene conversion , intergenic spacer , ITS , ITS, internally transcribed sequence , rDNA evolution , recombination , v, variable region and vc, variable/constant region
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2002-03-01
2020-04-02
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Intragenomic and intraspecific polymorphism of the 16S–23S rDNA internally transcribed sequences of Streptomyces ambofaciensThe GenBank accession numbers for the sequences reported in this paper can be found in Table 1.
Microbiology 148, 633 (2002); https://doi.org/10.1099/00221287-148-3-633
/content/journal/micro/10.1099/00221287-148-3-633
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