1887

Abstract

Genotypic characterization, based on the analysis of restriction fragment length polymorphism of the gene fragment PCR product ( PCR-RFLP), was performed on members of the former genus. PCR primers deduced from published gene sequences of allowed the amplification of an approximately 730 bp DNA fragment from each of the 19 species tested. Amplified fragments were compared using RFLP analysis with four endonucleases (I, fI, I and 1I), allowing the detection of characteristic patterns of RFLP products for most of the species. Between one and three specific RFLP groups were identified among most of the species tested (, , , , , , , , , , , , , , , , , subsp. , subsp. , subsp. and subsp. ). However, in two cases, and subsp. , 15 and 18 specific RFLP groups were detected, respectively. The variability of genetic patterns within these bacteria could be explained in terms of their geographic origin and/or wide host-range. The results indicated that PCR-RFLP analysis of the gene fragment is a useful tool for identification of species and subspecies belonging to the former genus, as well as for differentiation of strains within subsp. and

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2002-02-01
2020-09-30
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