1887

Abstract

The individual and synergistic contributions of two transcription factors, and , have been characterized with regard to adhesion to, and invasion of, human epithelia by . For this purpose two reconstructed tissue models were developed. A multi-layered model of human epidermis was used to simulate superficial infections of the skin, whereas a reconstructed human intestinal model was used to mimic the first steps of systemic infections. It was shown that deleted for both transcription factors and , in contrast to the congenic clinical isolate Sc5314, was neither able to adhere to, nor to penetrate, either of the model systems. A strain deleted for alone showed significant reduction in adhesion and was not able to penetrate through the stratum corneum. However, strains deleted for showed phenotypes paralleling the phenotypes of the clinical isolate Sc5314. Using different types of multi-layered human tissues reconstructed the individual contributions of Efg1p and Cph1p to two important virulence factors of , namely adhesion and invasion, could be defined.

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2002-02-01
2019-08-23
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