1887

Abstract

PCR amplicons of the Internal Transcribed Spacer (ITS) of the operon of three axenic OMF (oceanic, marine and freshwater) strains of ‘’ (WH7803, PCC 7001 and PCC 6307, respectively) differ greatly in length from that of the axenic subsp. PCC 9511, although these four cyanobacteria cluster relatively closely in phylogenetic trees inferred from 16S rRNA gene sequences. The ITSs of three strains (PCC 9511, PCC 6307 and PCC 7001) were sequenced and compared with those available for strains MED4 (CCMP 1378) and MIT9313 from genome sequencing projects. In spite of large differences in length, sequence and mean DNA base composition, conserved domains important for transcriptional antitermination and folding of the rRNA transcripts were identified in all ITSs. A new group-specific primer permitted ITS amplification even with non-axenic isolates of and one OMF-‘’ strain. isolates of the high-light-adapted clade (HL) differed from representatives of the low-light-adapted clade (LL) by the length of their ITS. Restriction fragment length polymorphism (RFLP) of the ITS amplicons revealed three subclusters among the HL strains. Size, sequence data and RFLP of the ITS amplicons will therefore be valuable markers for the identification of different genotypes and for their discrimination from other cyanobacterial relatives with which they often co-exist in oceanic ecosystems.

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2002-02-01
2019-10-17
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