1887

Abstract

The 23K chromosome was analysed by pulsed-field gel electrophoresis after digestion with the restriction enzymes I, I and I. The chromosome size was estimated to be 1845±80 kb. The use of I-I, specific for genes encoding 23S rRNAs, showed that seven loci were present, on 40% of the chromosome. The seven clusters were mapped and their orientation was determined, allowing the position of the replication origin to be estimated. Partial I-I digestions were used to construct a backbone and the different restriction fragments obtained with I, I and I were assembled to a physical map by Southern hybridization. Eleven gene clusters previously identified were mapped, as well as 25 new loci located randomly on the chromosome and 11 regions flanking the gene clusters. A total of 47 clusters were thus mapped on chromosome. The new loci were sequenced, allowing the identification of 73 complete or incomplete coding sequences. Among these 73 new genes of , the function of 36 could be deduced from their similarity to known genes described in databases. However, 10 genes had no homologues, 10 encoded proteins similar to proteins of unknown function and 17 were similar to hypothetical proteins.

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2002-02-01
2020-04-01
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