%0 Journal Article %A Weitnauer, G %A Gaisser, S %A Kellenberger, L %A Leadlay, P. F %A Bechthold, A %T Analysis of a C-methyltransferase gene (aviG1) involved in avilamycin biosynthesis in Streptomyces viridochromogenes Tü57 and complementation of a Saccharopolyspora erythraea eryBIII mutant by aviG1The GenBank accession number for the sequence reported in this paper is AF333038. %D 2002 %J Microbiology, %V 148 %N 2 %P 373-379 %@ 1465-2080 %R https://doi.org/10.1099/00221287-148-2-373 %K methyltransferase %K deoxysugar biosynthetic genes %K avilamycin A %K erythromycin %K ES-MS, electrospray mass spectrometry %I Microbiology Society, %X Streptomyces viridochromogenes Tü57 is the principal producer of avilamycin A. aviG1, a putative methyltransferase gene, was detected in the avilamycin biosynthetic gene cluster. To determine the function of aviG1, a targeted gene inactivation experiment was performed. The resulting chromosomal mutant, carrying an in-frame deletion in aviG1, was deficient in avilamycin production. aviG1 was used to complement an eryBIII mutant of the erythromycin A producer Saccharopolyspora erythraea [Gaisser, S., Bohm, G. A., Doumith, M., Raynal, M. C., Dhillon, N., Cortes, J. & Leadlay, P. F. (1998) R12 . Mol Gen Genet 258, 78–88]. The presence of erythromycin A in the culture supernatant of the complemented mutant indicated that L-mycarose biosynthesis could be restored and that AviG1 could take over the function of the C-methyltransferase EryBIII. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-2-373