1887

Abstract

Tü57 is the principal producer of avilamycin A. , a putative methyltransferase gene, was detected in the avilamycin biosynthetic gene cluster. To determine the function of , a targeted gene inactivation experiment was performed. The resulting chromosomal mutant, carrying an in-frame deletion in , was deficient in avilamycin production. was used to complement an mutant of the erythromycin A producer [Gaisser, S., Bohm, G. A., Doumith, M., Raynal, M. C., Dhillon, N., Cortes, J. & Leadlay, P. F. (1998) . 258, 78–88]. The presence of erythromycin A in the culture supernatant of the complemented mutant indicated that L-mycarose biosynthesis could be restored and that AviG1 could take over the function of the C-methyltransferase EryBIII.

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2002-02-01
2020-09-24
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