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Volume 148, Issue 12

Identification and functional expression of , a filamentous fungal gene involved in copper trafficking to the secretory pathway in Free

Abstract

In this study, cDNA and genomic clones encoding a homologue of the yeast gene anti-oxidant 1 () from the white-rot fungus , a basidiomycete known to produce several laccase isoenzymes involved in lignin degradation, were identified. This gene, named homologue (), encodes a protein of 79 kDa with 56% identity to the yeast Atx1p sequence. Two different alleles of were obtained that differed mainly in their intervening sequences and in a 425 nt insertion located 183 nt upstream of the transcription start site. is present as one copy per haploid nucleus in , as shown by Southern analysis. Expression of cDNA restored high-affinity iron uptake in a Δ yeast strain and oxygen sensitivity in a Δ Δ yeast strain, showing that is also a functional homologue of . The inability of to rescue the Δ phenotype on copper-deficient medium indicated that function is copper-dependent. Sequence analysis of the promoter revealed several motifs that were similar to the conserved motifs found in the copper-regulated metallothionein and Cu, Zn superoxide dismutase genes, and , of , and . In contrast to its yeast homologue , is induced under elevated copper concentrations in the medium (>025 μM CuSO) and repressed under copper starvation. The transcription of was analysed in response to copper and iron, and after adding xenobiotica. The results are discussed in relevance to laccase expression.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): BCS, bathocuproinedisulfonic acid , DABA, diaminobenzaldehyde , Ferrozine, 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′-disulfonic acid , IS, insertion sequence , laccase , metallochaperone , SOD, superoxide dismutase and white-rot fungi
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2002-12-01
2024-04-23
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Identification and functional expression of tahA, a filamentous fungal gene involved in copper trafficking to the secretory pathway in Trametes versicolor
Microbiology 148, 4049 (2002); https://doi.org/10.1099/00221287-148-12-4049
/content/journal/micro/10.1099/00221287-148-12-4049
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