1887

Abstract

The expression of six of the mammalian cell-entry () genes of the operon of has been described previously. In this study, data are presented for the expression of other mammalian cell-entry homologues (, and ) at the RNA level, as determined by RT-PCR. The stress responses of these genes and of other immunologically important antigens are also characterized with respect to the introduction of oxygen depletion. Analysis of the expression of the genes in relation to oxygen depletion revealed that they were expressed differentially. The RT-PCR results showed that , , (encoding the α-crystallin antigen Acr) and (encoding the early secretory antigenic target-6) were expressed throughout the cultivation period, whereas the expression of and was downregulated in the later stages of cultivation. This study gives new insights into the expression profiles of the different operons and the and genes in an model of dormant-like bacilli. Identification of the genes that are differentially expressed under aerobic conditions and under oxygen-limited conditions contributes to our understanding of the bacilli involved in latent tuberculosis.

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2002-12-01
2020-01-22
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