1887

Abstract

The post-transcriptional processing of tRNAs decorates them with a number of modified bases important for their biological functions. Queuosine, found in the tRNAs with GUN anticodons (Asp, Asn, His, Tyr), is an extensively modified base whose biosynthetic pathway is still unclear. In this study, it was observed that the tRNA from B105 (a B strain) migrated faster than that from CA274 (a K-12 strain) on acid urea gels. The organization of tRNA genes in B105 was found to be typical of the B strains. Subsequent analysis of tRNA and tRNA from several strains of on acid urea gels, and modified base analysis of tRNA preparations enriched for tRNA, showed that B105 lacked queuosine in its tRNAs. However, the lack of queuosine in tRNAs was not a common feature of all B strains. The and genes in B105 were shown to be functional by their ability to complement and mutant strains. These observations suggested a block at the step of the biosynthesis of preQ (or preQ) in the B105 strain. Interestingly, a multicopy vector harbouring a functional gene was toxic to B105 but not to CA274. Also, in mixed cultures, B105 was readily competed out by the CA274 strain. The importance of these observations and this novel strain ( B105) in unravelling the mechanism of preQ or preQ biosynthesis is discussed.

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2002-12-01
2020-11-25
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