1887

Abstract

Most pathogenic fungi have the ability to alternate between a unicellular yeast form and different filamentous forms (hyphae and pseudohyphae). This attribute is generally regarded as an important virulence factor and has also attracted attention because of its implications in the study of eukaryotic cell differentiation. To identify genes that are involved in the regulation of these events, chemical mutagenesis of the dimorphic yeast was performed and morphological mutants that were unable to form hyphal cells were isolated. Screening of a genomic DNA library for genes able to complement this defect led to the isolation of , a gene encoding a 14-3-3 protein and whose transcription levels are increased during the yeast-to-hypha transition. Remarkably, overexpression of was able to enhance pseudohyphae formation in a strain lacking functional but caused no visible effects in Δ and Δ cells, thus suggesting that is involved in the regulation of both hyphal and pseudohyphal growth in . The identification of , a gene encoding a second 14-3-3 protein (Bmh2p) that contains a 19 aa insertion absent in all other members of the 14-3-3 family, is also reported. Differently from , the transcription levels of do not show any apparent variation during the induction of hyphal growth, and its overexpression has no effects on cells lacking functional , or . Taken together, these observations suggest that, in spite of their high conservation, Bmh1p and Bmh2p have different cellular functions.

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2002-11-01
2019-08-23
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