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Volume 148, Issue 11

Copper- and zinc-containing superoxide dismutase (Cu/ZnSOD) is required for the protection of against oxidative stresses and the expression of its full virulence Free

Abstract

Copper- and zinc-containing superoxide dismutase (Cu/ZnSOD) is suspected to be one of the anti-oxidant enzymes and virulence determinants active in some pathogenic micro-organisms. To elucidate the role of Cu/ZnSOD in the major human fungal pathogen , its gene, designated , was disrupted by the URA-blaster technique. The resulting / mutant showed delayed hyphal growth on Spider medium but could still form hyphae on other solid media or in liquid media, particularly in response to serum. Moreover, the / mutant was more sensitive to menadione, a redox-cycling agent, than the isogenic wild-type strain, although it still showed an adaptive oxidative stress response. Furthermore, the / mutant cells exhibited slow growth in minimal medium when compared to the wild-type cells, but their growth was restored by the addition of lysine to the medium. Interestingly, cells lacking Cu/ZnSOD showed increased susceptibility to macrophage attack and had attenuated virulence in mice. Thus, these results suggest that Cu/ZnSOD is required for the protection of against oxidative stresses and for the full virulence of the organism to be expressed.

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Keyword(s): candidiasis , Cu/ZnSOD, copper- and zinc-containing SOD , FeSOD, iron-containing SOD , hyphal growth , lysine auxotrophy , MnSOD, manganese-containing SOD , ROS, reactive oxygen species , SG, synthetic glucose , SGU, SG plus 25 μg uridine ml−1 , SOD, superoxide dismutase and SOD1
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2002-11-01
2024-10-10
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/content/journal/micro/10.1099/00221287-148-11-3705
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Copper- and zinc-containing superoxide dismutase (Cu/ZnSOD) is required for the protection of Candida albicans against oxidative stresses and the expression of its full virulence
Microbiology 148, 3705 (2002); https://doi.org/10.1099/00221287-148-11-3705
/content/journal/micro/10.1099/00221287-148-11-3705
/content/journal/micro/10.1099/00221287-148-11-3705
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