1887

Abstract

The tetrathionate (Ttr) and thiosulfate (Phs) reductases of LT2, together with the polysulfide reductase (Psr) of , are unusual examples of enzymes containing a molybdopterin active-site cofactor since all formally catalyse sulfur–sulfur bond cleavage. This is in contrast to the oxygen or hydrogen transfer reactions exhibited by other molybdopterin enzymes. Here the catalytic specificity of Ttr and Phs has been compared using both physiological and synthetic electron-donor systems. Ttr is shown to catalyse reduction of trithionate but not sulfur or thiosulfate. In contrast, Phs cannot reduce tetrathionate or trithionate but allows whole cells to utilize elemental sulfur as an electron acceptor. Mechanisms are proposed by which the bacterium is able to utilize an insoluble sulfur substrate by means of reactions at the cytoplasmic rather than the outer membrane.

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2002-11-01
2019-10-14
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References

  1. Axley, M. J., Grahame, D. A. & Stadtman, T. C. ( 1990; ). Escherichia coli formate-hydrogen lyase. Purification and properties of the selenium-dependent formate dehydrogenase component. J Biol Chem 265, 18213-18218.
    [Google Scholar]
  2. Barrett, E. L. & Clark, M. A. ( 1987; ). Tetrathionate reduction and production of hydrogen sulfide from thiosulfate. Microbiol Rev 51, 192-205.
    [Google Scholar]
  3. Barrett, E. L. & Riggs, D. L. ( 1982; ). Evidence for a second nitrate reductase activity that is distinct from the respiratory enzyme in Salmonella typhimurium. J Bacteriol 150, 563-571.
    [Google Scholar]
  4. Casadaban, M. J. & Cohen, S. N. ( 1979; ). Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci USA 76, 4530-4533.[CrossRef]
    [Google Scholar]
  5. Clark, M. A. & Barrett, E. L. ( 1987; ). The phs gene and hydrogen sulfide production by Salmonella typhimurium. J Bacteriol 169, 2391-2397.
    [Google Scholar]
  6. Craske, A. & Ferguson, S. J. ( 1986; ). The respiratory nitrate reductase of Paracoccus denitrificans. Molecular characterization and kinetic properties. Eur J Biochem 158, 429-436.[CrossRef]
    [Google Scholar]
  7. Fong, C.-L. W., Heinzinger, N. K., Tongklan, S. & Barrett, E. L. ( 1993; ). Cloning of the phs genetic locus from Salmonella typhimurium and a role for a phs product in its own induction. J Bacteriol 175, 6368-6371.
    [Google Scholar]
  8. Hedderich, R., Klimmek, O., Kröger, A., Dirmeier, R., Keller, M. & Stetter, K. O. ( 1998; ). Anaerobic respiration with sulfur, polysulfide and disulfide. FEMS Microbiol Rev 22, 353-381.[CrossRef]
    [Google Scholar]
  9. Heinzinger, N. K., Fujimoto, S. Y., Clark, M. A., Moreno, M. S. & Barrett, E. L. ( 1995; ). Sequence analysis of the phs operon in Salmonella typhimurium and the contribution of thiosulfate reduction to anaerobic energy metabolism. J Bacteriol 177, 2813-2820.
    [Google Scholar]
  10. Hensel, M., Hinsley, A. P., Nikolaus, T., Sawers, G. & Berks, B. C. ( 1999; ). The genetic basis of tetrathionate respiration in Salmonella typhimurium. Mol Microbiol 32, 275-288.[CrossRef]
    [Google Scholar]
  11. Hille, R. ( 1996; ). The mononuclear molybdenum enzymes. Chem Rev 96, 2757-2816.[CrossRef]
    [Google Scholar]
  12. Huang, C. J. & Barrett, E. L. ( 1990; ). Identification and cloning of genes involved in anaerobic sulfite reduction by Salmonella typhimurium. J Bacteriol 172, 4100-4102.
    [Google Scholar]
  13. Kelly, D. P. & Wood, A. ( 1994; ). Synthesis and determination of thiosulphate and polythionates. Methods Enzymol 243, 475-501.
    [Google Scholar]
  14. Khangulov, S. V., Gladyshev, V. N., Dismukes, G. C. & Stadtman, T. C. ( 1998; ). Selenium-containing formate dehydrogenase H from Escherichia coli: a molybdopterin enzyme that catalyzes formate oxidation without oxygen transfer. Biochemistry 37, 3518-3528.[CrossRef]
    [Google Scholar]
  15. Klimmek, O., Kröger, A., Steudel, R. & Holdt, G. ( 1991; ). Growth of Wolinella succinogenes with polysulphide as terminal acceptor of phosphorylative electron transport. Arch Microbiol 155, 177-182.[CrossRef]
    [Google Scholar]
  16. Klimmek, O., Kreis, V., Klein, C., Simon, J., Wittershagen, A. & Kröger, A. ( 1998; ). The function of the periplasmic Sud protein in polysulfide respiration of Wolinella succinogenes. Eur J Biochem 253, 263-269.[CrossRef]
    [Google Scholar]
  17. Maloy, S. R., Stewart, V. J. & Taylor, R. K. (1996). Genetic Analysis of Pathogenic Bacteria. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  18. Moser, D. P. & Nealson, K. H. ( 1996; ). Growth of the facultative anaerobe Shewanella putrefaciens by elemental sulfur reduction. Appl Environ Microbiol 62, 2100-2105.
    [Google Scholar]
  19. Pollock, M. R. & Knox, R. ( 1943; ). Bacterial reduction of tetrathionate. Biochem J 37, 476-481.
    [Google Scholar]
  20. Pope, N. R. & Cole, J. A. ( 1982; ). Generation of a membrane-potential by one of two independent pathways for nitrite reduction by Escherichia coli. J Gen Microbiol 128, 219-222.
    [Google Scholar]
  21. Price-Carter, M., Tingey, J., Bobik, T. A. & Roth, J. R. ( 2001; ). The alternative electron acceptor tetrathionate supports B12-dependent anaerobic growth of Salmonella enterica serovar Typhimurium on ethanolamine or 1,2-propanediol. J Bacteriol 183, 2463-2475.[CrossRef]
    [Google Scholar]
  22. Riggs, D. L., Tang, J. S. & Barrett, E. L. ( 1987; ). Thiosulfate reductase as a chlorate reductase in Salmonella typhimurium. FEMS Microbiol Lett 44, 427-430.[CrossRef]
    [Google Scholar]
  23. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  24. Schauder, R. & Kröger, A. ( 1993; ). Bacterial sulfur respiration. Arch Microbiol 159, 491-497.[CrossRef]
    [Google Scholar]
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