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Volume 148, Issue 11

The inner-core lipopolysaccharide biosynthetic gene: function and genetic distribution among some

bThe GenBank accession number for the gene sequences of CECT170, R102 and CECT684 reported in this paper are AY075039, AY075041 and AY075040, respectively.

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Abstract

To determine the function of the gene in the biosynthesis of the inner-core LPS of , a non-polar mutant has been constructed. Data obtained from the comparative chemical analysis of LPS samples obtained from the wild-type, the mutant strain and the complemented mutant demonstrated that the gene is involved in substitution of α-L--D--heptopyranose I (L,D-HepI) at the O-4 position by a β-D-glucopyranose (β-D-Glc) residue. In addition, DNA amplification and nucleotide sequence determination studies revealed that homologues located between the and genes are present in clinical isolates of containing the structure β-D-Glc-(1→4)-α-L,D-HepI (, and ), as well as in strains of and of unknown LPS-core structures. Complementation studies using non-polar mutants prove that all the homologues perform the same function. Furthermore, , and non-polar mutants showed reduced adhesion and pathogenicity. In addition, the and mutants showed reduced swarming motility and ability to form biofilms . All these characteristics were rescued by reintroduction of the gene independently of its origin. An easy DNA amplification method to detect this gene was established, which also helps in finding the potential presence of this structural feature [β-D-Glc-(1→4)-α-L,D-HepI] in the inner-core LPS of members with unknown LPS-core structures.

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  • Accepted:
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Keyword(s): BATH, bacterial adherence to hydrocarbons , cross-complementation , GlcN(p), glucosamine , HIC, hydrophobic interaction chromatography , Kdo(p), 3-deoxy-D-manno-octulopyranosonic acid , L,D-Hep(p), α-L-glycero-D-manno-heptopyranose , LPS , NHS, non-immune human serum , non-polar mutant characterization , waaE homologues , α-D-Gal(p)A, α-D-galacturonic acid and β-D-Glc(p), β-D-glucopyranose
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2002-11-01
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The inner-core lipopolysaccharide biosynthetic waaE gene: function and genetic distribution among some EnterobacteriaceaebbThe GenBank accession number for the waaE gene sequences of P. mirabilis CECT170, Y. enterocolitica R102 and Ent. aerogenes CECT684 reported in this paper are AY075039, AY075041 and AY075040, respectively.
Microbiology 148, 3485 (2002); https://doi.org/10.1099/00221287-148-11-3485
/content/journal/micro/10.1099/00221287-148-11-3485
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