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Volume 148, Issue 11

Novel insights into the interplay between peripheral reactions encoded by genes and the chlorocatechol pathway encoded by genes for the degradation of chlorobenzoates by JMP134 Free

Abstract

Many bacteria can grow on chloroaromatic pollutants because they can transform them into chlorocatechols, which are further degraded by enzymes of a specialized -cleavage pathway. JMP134 is able to grow on 3-chlorobenzoate by using two pJP4-encoded, -cleavage chlorocatechol degradation gene clusters ( and ). Very little is known about the acquisition of new catabolic genes encoding enzymes that lead to the formation of chlorocatechols in JMP134. The effect on the catabolic properties of an JMP134 derivative that received the – gene module, encoding the -regulated expression of the broad-substrate-range toluate 1,2-dioxygenase () and the 1,2-dihydro-1,2-dihydroxytoluate dehydrogenase () from pWW0, which allows the transformation of 4-chlorobenzoate into 4-chlorocatechol, was studied. Such a derivative could efficiently grow on 4-chlorobenzoate. Unexpectedly, this derivative also grew on 3,5-dichlorobenzoate, a substrate for XylXYZL but not an inducer of the XylS regulatory protein. The ability to grow on 4-chlorobenzoate or 3,5-dichlorobenzoate was also observed in derivatives of strain JMP134 containing the gene module but lacking , indicating the presence of an -like element in with an inducer profile different from that of the pWW0-encoded regulator. Growth on 4-chlorobenzoate was also observed after introduction of the gene module into strain JMP222, a JMP134 derivative lacking pJP4, but only if multiple copies of or were present. However, only the derivative containing multiple copies of was able to grow on 3,5-dichlorobenzoate. These observations indicate that although the acquisition of new catabolic genes actually enhances the catabolic abilities of JMP134, these new properties are strongly influenced by the dosage of the genes, the presence of a chromosomal -like regulatory element and the different contributions of the gene clusters.

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Keyword(s): 2,4-D, 2,4-dichlorophenoxyacetate , 3,5-DCB, 3,5-dichlorobenzoate , 3-CB, 3-chlorobenzoate , 3-MB, 3-methylbenzoate , 4-CB, 4-chlorobenzoate , 4-MB, 4-methylbenzoate , AraC/XylS regulatory family , CB, chlorobenzoate , chloroaromatic compounds , gene acquisition , gene dosage and maleylacetate reductase
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2002-11-01
2024-04-25
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Novel insights into the interplay between peripheral reactions encoded by xyl genes and the chlorocatechol pathway encoded by tfd genes for the degradation of chlorobenzoates by Ralstonia eutropha JMP134
Microbiology 148, 3431 (2002); https://doi.org/10.1099/00221287-148-11-3431
/content/journal/micro/10.1099/00221287-148-11-3431
/content/journal/micro/10.1099/00221287-148-11-3431
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