@article{mbs:/content/journal/micro/10.1099/00221287-148-10-3265, author = "Shiomi, Daisuke and Homma, Michio and Kawagishi, Ikuro", title = "Intragenic suppressors of a mutation in the aspartate chemoreceptor gene that abolishes binding of the receptor to methyltransferase", journal= "Microbiology", year = "2002", volume = "148", number = "10", pages = "3265-3275", doi = "https://doi.org/10.1099/00221287-148-10-3265", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-10-3265", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "revertant", keywords = "histidine kinase", keywords = "chemotaxis", keywords = "methylation", keywords = "adaptation", abstract = "In the chemotaxis of Escherichia coli, receptor methylation is the key process of adaptation. The methyltransferase CheR binds to the carboxy-terminal NWETF sequence of major chemoreceptors. The substitution of Ala for Trp of this sequence (W550A) of the aspartate chemoreceptor (Tar) abolishes its CheR-binding ability. In this study, six independent intragenic suppressors of the mutation were isolated. They were divided into two classes. Tar carrying the class I suppressors (G278A-L488M, T334A, G278A, G278C and A398T) showed signal biases toward tumbling, corresponding to increased activities of the receptor-associated histidine kinase CheA. These suppressors further reduced the unstimulated methylation level of Tar-W550A, but allowed slight but significant stimulation of methylation by aspartate. Some other CheA-activating mutations were also found to serve as class I suppressors. These results suggest that the class I suppressors compensate for the signal bias of Tar-W550A caused by its low methylation level and that the NWETF sequence is required primarily to maintain an appropriate level of methylation by increasing the local concentration of CheR around the receptor. The class II suppressor was a mutation in the termination codon (Op554W) resulting in the addition of 11 residues containing an xWxxF motif. This revertant Tar supported chemotaxis and was methylated almost as effectively as wild-type Tar. This effect was reversed by introducing a mutation in the xWxxF motif. These results reinforce the importance of the xWxxF motif and suggest that the motif does not have to be located at the extreme carboxy terminus.", }