1887

Abstract

The Sca permease in the oral bacterium is a member of a family of ATP-binding cassette (ABC)-type transporters for manganese (Mn) and related cations that are associated with streptococcal virulence in a number of infection models. Since Mn has a protective function against oxidative damage in a variety of bacteria, we have investigated the role of Sca permease in oxidative stress tolerance in . A single Mn-dependent superoxide dismutase (SOD), encoded by , is expressed by and was >10-fold up-regulated under oxidative stress conditions. Inactivation of resulted in increased susceptibility of cells to growth inhibition by dioxygen (O), and to killing by paraquat (a superoxide anion generator) and by hydrogen peroxide (HO). Expression of thiol peroxidase, encoded by the gene located immediately downstream of the operon, was also up-regulated under oxidative conditions. Inactivation of led to increased susceptibility of cells to HO, but not to O or paraquat. In low-Mn medium (001 μM Mn) and genes were transcriptionally down-regulated, SOD activity was reduced and cells were more sensitive to growth inhibition by O. A Sca permease-deficient () mutant showed further reduced SOD activity and hypersensitivity to O in medium containing <01 μM Mn. These results demonstrate that the Sca (Mn) permease in is essential for protection against oxidative stress.

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2002-10-01
2024-03-28
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