1887

Abstract

Group B streptococcus (GBS) is surrounded by a capsule. However, little is known about peptidoglycan metabolism in these bacteria. In the present study, a 65 kDa protein was isolated from the culture supernatant of GBS and N-terminally sequenced, permitting isolation of the corresponding gene, termed . The gene was located close to another gene, designated , and reverse transcription-PCR revealed a bicistronic transcriptional organization for both genes. The Bsp protein was detected in the culture supernatant from 31 tested clinical isolates of GBS, suggesting a wide distribution of Bsp in these bacteria. Overexpression of resulted in lens-shaped GBS cells, indicating a role for in controlling cell morphology. Insertional disruption of resulted in a reduction of the L-alanine content of the peptidoglycan, suggesting that is involved in the incorporation of L-alanine residues in the interpeptide chain of the peptidoglycan of GBS.

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2002-10-01
2019-10-16
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