1887

Abstract

A gene originally identified by signature-tagged mutagenesis as being required for virulence was cloned, sequenced and named . Hydropathy profiles revealed that SvrA is likely to be membrane associated, having two regions with six membrane-spanning domains, the regions separated by an extended hydrophilic loop. When compared with the wild-type strain, an mutant expressed greatly reduced amounts of α-, β- and δ-toxins and an increased amount of protein A. Toxin production by the mutant strain was restored to wild-type levels when complemented with a plasmid expressing the gene. Northern hybridization with probes specific for (encoding α-toxin) and (encoding protein A) showed that the mutant strain was affected in the transcription of these genes. mRNA was present in wild-type and strains, but mRNA and RNAIII were absent in the mutant strain. Virulence studies suggested that the attenuation of the mutant was probably due to its direct or indirect effect on the regulon. These results indicate that is required for the expression of and RNAIII transcripts and is therefore a new component of the regulatory network controlling virulence gene expression in .

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2002-10-01
2020-01-21
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