1887

Microbiology Society logo

Volume 148, Issue 10

The HPLC-double-cluster pattern of some strains is due to their dicarboxy-mycolate content Free

Abstract

The mycolic acids of several strains of were examined by chromatographic and spectroscopic techniques. Both HPLC and TLC revealed two patterns of mycolates among the strains studied. As determined by TLC, one pattern was composed of α-, methoxy- and keto-mycolates; the other was composed of these mycolates plus an additional component, which was identified as dicarboxy-mycolates. The dicarboxy-mycolates were only found in those strains that displayed a so-called HPLC-double-cluster pattern. Detailed structural analyses of the dicarboxy-mycolates indicated that these compounds contained predominantly 61–65 carbon atoms (C was the major component) and a 1,2-disubstituted cyclopropane ring. Thus, the dicarboxy-mycolate content of strains of determines their HPLC pattern. In spite of the differences in their HPLC patterns, and although they belonged to different PCR-restriction length polymorphism clusters, all of the strains examined in this study were closely related on the basis of the structural features of their α-, keto- and methoxy-mycolates; the predominant α-mycolates contained two -1,2-disubstituted cyclopropane rings, the major keto-mycolates contained a -1,2-disubstituted cyclopropane ring and the methoxy-mycolates contained one - or one -1,2-disubstituted cyclopropane ring. It is noteworthy that the strains containing dicarboxy-mycolates also displayed significant amounts of α-mycolates that contained one -1,2-disubstituted cyclopropane ring and one double bond. The results obtained in this study demonstrate heterogeneity among strains.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): EI-MS, electron-impact MS , FAB-MS, fast-atom bombardment MS , Gen-Probe, Gen-Probe Rapid Diagnostic System , high-performance liquid chromatography , M. gordonae , mycolic acids and PRA, PCR-restriction length polymorphism analysis
© Microbiology Society
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-148-10-3119
2002-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/148/10/1483119a.html?itemId=/content/journal/micro/10.1099/00221287-148-10-3119&mimeType=html&fmt=ahah

References

  1. Barry C. E. 3rd, Lee R. E, Mdluli K, Sampson A. E, Schroeder B. G, Slayden R. A., Yuan Y. 1998; Mycolic acids: structure, biosynthesis and physiological functions. Prog Lipid Res 37:143–179
     [Google Scholar]
  2. Bernard E, Michiels J. F, Pinier Y, Bourdet J. F., Dellamonica P. 1992; Disseminated infection as a result of Mycobacterium gordonae in an AIDS patient. AIDS 6:1217–1218
     [Google Scholar]
  3. Butler W. R., Guthertz L. S. 2001; Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species. Clin Microbiol Rev 14:704–726
     [Google Scholar]
  4. Butler W. R, Jost K. C. Jr, Kilburn J. O. 1991; Identification of mycobacteria by high-performance liquid chromatography. J Clin Microbiol 29:2468–2472
     [Google Scholar]
  5. Cage G. D. 1992; High-performance liquid chromatography patterns of Mycobacterium gordonae mycolic acids. J Clin Microbiol 30:2402–2407
     [Google Scholar]
  6. Clague H, Hopkins C. A, Roberts C., Jenkins P. A. 1985; Pulmonary infection with Mycobacterium gordonae in the presence of bronchial carcinoma. Tubercle 66:61–63
     [Google Scholar]
  7. Daffé M, Lanéelle M. A, Promé D., Asselineau C. 1981; Étude des lipides de Mycobacterium gordonae comparativement à ceux de M. leprae et de quelques mycobactéries scotochromogènes. Ann Microbiol (Paris 132B:3–12
     [Google Scholar]
  8. Daffé M, Lanéelle M. A, Asselineau C, Lévy-Frebault V., David H. 1983; Intérêt taxonomique des acides gras des mycobactéries: proposition d’une methode d’analyse. Ann Microbiol (Paris) 134B:241–256
     [Google Scholar]
  9. Draper P, Dobson G, Minnikin D. E., Minnikin S. M. 1982; The mycolic acids of Mycobacterium leprae harvested from experimentally infected nine-banded armadillos. Ann Microbiol (Paris 133:39–47
     [Google Scholar]
  10. Glickman S. E, Kilburn J. O, Butler W. R., Ramos L. S. 1994; Rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a Mycobacterium library. J Clin Microbiol 32:740–745
     [Google Scholar]
  11. Kirschner P., Böttger E. C. 1992; Microheterogeneity within rRNA of Mycobacterium gordonae . J Clin Microbiol 30:1049–1050
     [Google Scholar]
  12. Lanéelle M. A., Lanéelle G. 1970; Structure d’acides mycoliques et d’un intermediaire dans la biosynthèse d’acides mycoliques dicarboxyliques. Eur J Biochem 12:296–300
     [Google Scholar]
  13. Laval F, Lanéelle M. A, Déon C, Monsarrat B., Daffé M. 2001; Accurate molecular mass determination of mycolic acids by MALDI-TOF mass spectrometry. Anal Chem 73:4537–4544
     [Google Scholar]
  14. Le Dantec C, Duguet J.-P, Montiel A, Dumoutier N, Dubrou S., Vincent V. 2002; Chlorine disinfection of atypical mycobacteria isolated from a water distribution system. Appl Environ Microbiol 68:1025–1032
     [Google Scholar]
  15. Leoni E, Legnani P, Mucci M. T., Pirani R. 1999; Prevalence of mycobacteria in a swimming pool environment. J Appl Microbiol 87:683–688
     [Google Scholar]
  16. London R. D, Damsker B, Neibart E. P, Knorr B., Bottone E. J. 1988; Mycobacterium gordonae : an unusual peritoneal pathogen in a patient undergoing continuous ambulatory peritoneal dialysis. Am J Med 85:703–704
     [Google Scholar]
  17. Luquin M, Roussel J, López-Calahorra F, Lanéelle G, Ausina V., Lanéelle M. A. 1990; A novel mycolic acid in a Mycobacterium sp. from the environment. Eur J Biochem 192:753–759
     [Google Scholar]
  18. Luquin M, Ausina V, López-Calahorra F, Belda F, Garcı́a-Barceló M, Celma C., Prats G. 1991; Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria. J Clin Microbiol 29:120–130
     [Google Scholar]
  19. Minnikin D. E. 1982; Lipids: complex lipids, their chemistry, biosynthesis and roles. In The Biology of the Mycobacteria pp 95–184 Edited by Ratledge C, Stanford J. L. London: Academic Press;
     [Google Scholar]
  20. Minnikin D. E, Minnikin S. M., Goodfellow M. 1982; The oxygenated mycolic acids of Mycobacterium fortuitum , M. farcinogenes and M. senegalense . Biochim Biophys Acta 712:616–620
     [Google Scholar]
  21. Minnikin D. E, Minnikin S. M, Parlett J. H, Goodfellow M., Magnusson M. 1984; Mycolic acid patterns of some species of Mycobacterium . Arch Microbiol 139:225–231
     [Google Scholar]
  22. Minnikin D. E, Minnikin S. M, Parlett J. H., Goodfellow M. 1985a; Mycolic acid patterns of some rapidly-growing species of Mycobacterium . Zentralbl Bakteriol Mikrobiol Hyg Ser A 259:446–460
     [Google Scholar]
  23. Minnikin D. E, Dobson G, Goodfellow M, Draper P., Magnusson M. 1985b; Quantitative comparison of the mycolic and fatty acid compositions of Mycobacterium leprae and Mycobacterium gordonae . J Gen Microbiol 131:2013–2021
     [Google Scholar]
  24. Papapetropoulou M, Tsintzou A., Vantarakis A. 1997; Environmental mycobacteria in bottled table waters in Greece. Can J Microbiol 43:499–502
     [Google Scholar]
  25. Plikaytis B. B, Plikaytis B. D, Yakrus M. A, Butler W. R, Woodley C. L, Silcox V. A., Shinnick T. M. 1992; Differentiation of slowly growing Mycobacterium species including Mycobacterium tuberculosis , by gene amplification and restriction fragment length polymorphism analysis. J Clin Microbiol 30:1815–1822
     [Google Scholar]
  26. Rusconi S, Gori A, Vago L, Marchetti G., Franzetti F. 1997; Cutaneous infection caused by Mycobacterium gordonae in a human immunodeficiency virus-infected patient receiving antimycobacterial treatment. Clin Infect Dis 25:1490–1491
     [Google Scholar]
  27. Telenti A, Marchesi F, Balz M, Bally F, Böttger E. C., Bodmer T. 1993; Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31:175–178
     [Google Scholar]
  28. Thibert L., Lapierre S. 1993; Routine application of high-performance liquid chromatography for identification of mycobacteria. J Clin Microbiol 31:1759–1763
     [Google Scholar]
  29. Torkko P, Suomalainen S, Iivanainen E. 7 other authors 2001; Characterization of Mycobacterium bohemicum isolated from human, veterinary, and environmental sources. J Clin Microbiol 39:207–211
     [Google Scholar]
  30. Tsukamura M. 1967; Identification of mycobacteria. Tubercle 48:311–338
     [Google Scholar]
  31. Valero-Guillén P. L, Pacheco F., Martı́n-Luengo F. 1985; Fatty acid composition and mycolic acid pattern of some chromogenic mycobacteria. J Appl Bacteriol 59:113–126
     [Google Scholar]
  32. Vogiatzakis E, Stefanou S, Skroubelou A, Anagnostou S, Marinis E., Matsiota-Bernard P. 1998; Molecular markers for the investigation of Mycobacterium gordonae epidemics. J Hosp Infect 38:217–222
     [Google Scholar]
  33. Walton D. T., Valesco M. 1991; Identification of Mycobacterium gordonae from culture by the Gen-Probe Rapid Diagnostic System: evaluation of 218 isolates and potential sources of false-negative results. J Clin Microbiol 29:1850–1854
     [Google Scholar]
  34. Watanabe M, Ohta A, Sasaki S., Minnikin D. E. 1999; Structure of a new glycolipid from the Mycobacterium avium – Mycobacterium intracellulare complex. J Bacteriol 181:2293–2297
     [Google Scholar]
  35. Watanabe M, Aoyagi Y, Ridell M., Minnikin D. E. 2001; Separation and characterization of individual mycolic acids in representative mycobacteria. Microbiology 147:1825–1837
     [Google Scholar]
  36. Wayne L. G., Kubica G. P. 1986; Genus Mycobacterium Lehmann and Neumann 1896, 363AL . In Bergey’s Manual of Systematic Bacteriology pp 1436–1457 Edited by Sneath P. H. A, Mair N. S, Sharpe M. E, Holt J. G. Baltimore, MD: Williams & Wilkins;
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-148-10-3119
Loading
The HPLC-double-cluster pattern of some Mycobacterium gordonae strains is due to their dicarboxy-mycolate content
Microbiology 148, 3119 (2002); https://doi.org/10.1099/00221287-148-10-3119
/content/journal/micro/10.1099/00221287-148-10-3119
/content/journal/micro/10.1099/00221287-148-10-3119
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error