1887

Abstract

mAb CS-35 is representative of a large group of antibodies with similar binding specificities that were generated against the lipopolysaccharide, lipoarabinomannan (LAM), and which cross-reacted extensively with LAMs from and other mycobacteria. That this antibody also cross-reacts with the arabinogalactan (AG) of the mycobacterial cell wall, suggesting that it recognizes a common arabinofuranosyl (Ara)-containing sequence in AG and LAM, is demonstrated. The antibody reacted more avidly with ‘AraLAM’ (LAM with naked Ara termini) compared to ‘ManLAM’ (in which many Ara termini are capped with mannose residues) and mycolylarabinogalactan–peptidoglycan complex (in which the terminal Ara units are substituted with mycolic acids). Neither did the antibody bind to AG from knock-out mutants deficient in the branched hexa-Ara termini of AG. These results indicate that the terminal Ara residues of mycobacterial arabinan are essential for binding. Competitive ELISA using synthetic oligosaccharides showed that the branched hexa-Ara methyl glycoside [β-D-Ara-(1→2)-α-D-Ara-(1-)-(3 and 5)-α-D-Ara-(1→5)-α-D-Ara-OCH] was the best competitor among those tested. The related linear methyl glycoside, β-D-Ara-(1→2)-α-D-Ara-(1→5)-α-D-Ara-(1→5)-α-D-Ara-OCH, representing one linear segment of the branched hexa-Ara, was less effective and the other linear tetrasaccharide, β-D-Ara-(1→2)-α-D-Ara-(1→3)-α-D-Ara-(1→5)-α-D-Ara-OCH, was ineffective. The combined results suggest that the minimal epitope recognized by antibody CS-35 encompasses the β-D-Ara-(1→2)-α-D-Ara-(1→5)-α-D-Ara-(1→5)-α-D-Ara within the branched hexa-Ara motif of mycobacterial arabinans, whether present in LAM or AG.

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2002-10-01
2019-10-16
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