1887

Microbiology

Volume 148, Issue 10

Synthesis of an unusual polar glycopeptidolipid in glucose-limited culture of Free

Abstract

There has been a general understanding that produces only apolar glycopeptidolipid (GPL), similar in structure to serovar non-specific GPL of . In this study, synthesis of polar GPL in carbon-starved is reported. Mass spectrometric analysis suggests the polar GPL to be a hyperglycosylated species. The earlier structural studies of polar GPLs from have invariably shown the presence of an oligosaccharide appendage to D-allo-Thr. However, a further chemical analysis using β-elimination of the newly found polar GPL in shows that the molecule still contains a monosaccharide at the D-allo-Thr, thus suggesting a new form of polar GPL.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 6-dTal, 6-deoxy talose , carbon starvation , ESI-MS, electrospray ionization mass spectrometry , GPL, glycopeptidolipid , MALDI-TOF, matrix assisted laser desorption ionization-time of flight , nsGPL, non-specific GPL and polar glycopeptidolipid
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2002-10-01
2019-10-19
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Synthesis of an unusual polar glycopeptidolipid in glucose-limited culture of Mycobacterium smegmatis
Microbiology 148, 3039 (2002); https://doi.org/10.1099/00221287-148-10-3039
/content/journal/micro/10.1099/00221287-148-10-3039
/content/journal/micro/10.1099/00221287-148-10-3039
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