RT Journal Article SR Electronic(1) A1 Verhamme, Daniƫl T A1 Arents, Jos C A1 Postma, Pieter W A1 Crielaard, Wim A1 Hellingwerf, Klaas JYR 2002 T1 Investigation of in vivo cross-talk between key two-component systems of Escherichia coli JF Microbiology, VO 148 IS 1 SP 69 OP 78 DO https://doi.org/10.1099/00221287-148-1-69 PB Microbiology Society, SN 1465-2080, AB Intracellular signal transfer in bacteria is dominated by phosphoryl transfer between conserved transmitter and receiver domains in regulatory proteins of so-called two-component systems. Escherichia coli contains 30 such systems, which allow it to modulate gene expression, enzyme activity and the direction of flagellar rotation. The authors have investigated whether, and to what extent, these separate systems form (an) interacting network(s) in vivo, focussing on interactions between four major systems, involved in the responses to the availability of phosphorylated sugars (Uhp), phosphate (Pho), nitrogen (Ntr) and oxygen (Arc). Significant cross-talk was not detectable in wild-type cells. Decreasing expression levels of succinate dehydrogenase (reporting Arc activation), upon activation of the Pho system, appeared to be independent of signalling through PhoR. Cross-talk towards NtrC did occur, however, in a ntrB deletion strain, upon joint activation of Pho, Ntr and Uhp. UhpT expression was demonstrated when cells were grown on pyruvate, through non-cognate phosphorylation of UhpA by acetyl phosphate., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-1-69