1887

Abstract

pSAM2 is integrated into the chromosome through site-specific recombination between the element () and the chromosomal () site. The 43 kDa integrase protein encoded by pSAM2 catalyses this recombination event. Tools have been developed to study site-specific recombination in . studies showed that a 360 bp fragment of is required for efficient site-specific recombination and that can be provided . pSAM2 integrase was purified and overexpressed in and Int binding at the site was studied. DNaseI footprinting revealed two sites that bind integrase strongly and appear to be symmetrical with regard to the core site. These two P1/P2 arm-type sites both contain a 17 bp motif that is identical except at one position, GTCACGCAG(A/T)TAGACAC. P1 and P2 are essential for site-specific recombination.

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2002-01-01
2020-04-05
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