1887

Abstract

(formerly ) can perform malolactic fermentation, converting L-malate to L-lactate and carbon dioxide, in wines. The energy and redox potential required to support the growth of the micro-organism are supplied mainly by the consumption of carbohydrates via the heterolactic pathway. In the first steps of hexose metabolism two molecules of NAD(P) are consumed, which must be regenerated in later reactions. The aim of this work was to test if aerobic growth of promotes higher cell yields than anaerobic conditions, as has been shown for other lactic acid bacteria. M42 was found to grow poorly under aerobic conditions with glucose as the only carbohydrate in the medium. It was demonstrated that O inactivates the enzymes of the ethanol-forming pathway, one of the two pathways which reoxidizes NAD(P) cofactors in the heterolactic catabolism of glucose. These results suggest that the regeneration of cofactors is the limiting factor for the aerobic consumption of glucose. When external electron acceptors, such as fructose or pyruvate, were added to glucose-containing culture medium the growth of was stimulated slightly; fructose was converted to mannitol, oxidizing two molecules of NAD(P)H, and pyruvate was transformed to lactate, enabling the regeneration of NAD. The addition of cysteine seemed to suppress the inactivation of the ethanol-forming pathway enzymes by O, enabling glucose consumption in aerobic conditions to reach similar rates to those found in anaerobic conditions.

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2002-01-01
2019-10-23
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References

  1. Anders, R. F., Hogg, D. M. & Jago, G. R. ( 1970; ). Formation of hydrogen peroxide by group N streptococci and its effect on their growth and metabolism. Appl Environ Microbiol 19, 608-612.
    [Google Scholar]
  2. Bruinenberg, P. G., de Roo, G. & Limsowtin, K. Y. ( 1997; ). Purification and characterization of cystathione γ-lyase from Lactococcus lactis subsp. cremoris SK11: possible role in flavor compound formation during cheese maturation. Appl Environ Microbiol 63, 561-566.
    [Google Scholar]
  3. Caspritz, G. & Radler, F. ( 1983; ). Malolactic enzyme of Lactobacillus plantarum. J Biol Chem 258, 4907-4910.
    [Google Scholar]
  4. Cogan, T. M. & Jordan, K. N. ( 1994; ). Metabolism of Leuconostoc bacteria. J Dairy Sci 77, 2704-2717.[CrossRef]
    [Google Scholar]
  5. Cogan, J. F., Walsh, D. & Condon, S. ( 1989; ). Impact of aeration on the metabolic end-products formed from glucose and galactose by Streptococcus lactis. J Appl Bacteriol 66, 77-84.[CrossRef]
    [Google Scholar]
  6. Diviès, C., Frey, L., Hubert, J. C. & de Roissart, H. ( 1994; ). Métabolisme d’autres substrats carbonés par les bactéries lactiques. In Bactéries Lactiques , pp. 291-307. Edited by H. de Roissart & F. M. Luquet. Uriage, France:Lorica.
  7. Firme, M. P., Leitão, M. C. & San Romão, M. V. ( 1994; ). The metabolism of sugar and malic acid by Leuconostoc oenos: effect of malic acid, pH and aeration conditions. J Appl Bacteriol 76, 173-181.[CrossRef]
    [Google Scholar]
  8. Ito, S., Hashiba, H. & Eguchi, Y. ( 1974; ). Adaptative control of the ethanol-forming system in heterolactic acid bacteria. J Biochem 75, 577-581.
    [Google Scholar]
  9. Ito, S., Kobayashi, T., Ohta, Y. I. & Akiyama, Y. ( 1983; ). Inhibition of glucose catabolism by aeration in Leuconostoc mesenteroides. J Ferment Technol 61, 353-358.
    [Google Scholar]
  10. Kelly, A. F. & Patchett, R. A. ( 1996; ). Lactate and acetate production in Listeria innocua. Lett Appl Microbiol 23, 125-128.[CrossRef]
    [Google Scholar]
  11. Klingenberg, H. U. (1985). Methods of Enzymatic Analysis. 3rd edn, vol. VII. New York: Academic Press.
  12. Lucey, C. A. & Condon, S. ( 1986; ). Active role of oxygen and NADH oxidase in growth and energy metabolism of Leuconostoc. J Gen Microbiol 132, 1789-1796.
    [Google Scholar]
  13. Maicas, S., González-Cabo, P., Ferrer, S. & Pardo, I. ( 1999; ). Production of Oenococcus oeni biomass to induce malolactic fermentation in wine by control of pH and substrate addition. Biotechnol Lett 21, 349-353.[CrossRef]
    [Google Scholar]
  14. Murphy, M. G. & Condon, S. ( 1984; ). Correlation of oxygen utilization and hydrogen peroxide accumulation with oxygen induced enzymes in Lactobacillus plantarum cultures. Arch Microbiol 138, 44-48.[CrossRef]
    [Google Scholar]
  15. Nuraida, L., Grigolava, I., Owens, J. D. & Campbell-Platt, G. ( 1992; ). Oxygen and pyruvate as external electron acceptors for Leuconostoc spp. J Appl Bacteriol 72, 517-522.[CrossRef]
    [Google Scholar]
  16. Pardo, I. & Zúñiga, M. ( 1992; ). Lactic acid bacteria in spanish red rosé and white musts and wines under cellar conditions. J Food Sci 57, 392-405.[CrossRef]
    [Google Scholar]
  17. Plihon, F., Taillandier, P. & Strahaiano, P. ( 1995; ). Oxygen effect on batch cultures of Leuconostoc mesenteroides: relationship between oxygen uptake, growth and end-products. Appl Microbiol Biotechnol 43, 117-122.[CrossRef]
    [Google Scholar]
  18. Sakamoto, M. & Komagata, K. ( 1996; ). Aerobic growth of and activities of NADH oxidase and NADH peroxidase in lactic acid bacteria. J Ferment Bioeng 82, 210-216.[CrossRef]
    [Google Scholar]
  19. Salou, P., Loubière, P. & Pareilleux, A. ( 1994; ). Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose. Appl Environ Microbiol 60, 1459-1466.
    [Google Scholar]
  20. Veiga-da-Cunha, M., Firme, P., San Romão, V. & Santos, H. ( 1992; ). Application of 13C nuclear magnetic resonance to elucidate the unexpected biosynthesis of erythritol by Leuconostoc oenos. Appl Environ Microbiol 58, 2271-2279.
    [Google Scholar]
  21. Veiga-da-Cunha, M., Santos, H. & van Schaftingen, E. ( 1993; ). Pathway and regulation of erythritol formation in Leuconostoc oenos. J Bacteriol 157, 3941-3948.
    [Google Scholar]
  22. Warriner, K. S. R. & Morris, J. G. ( 1995; ). The effects of aeration on the bioreductive abilities of some heterofermentative lactic acid bacteria. Lett Appl Microbiol 20, 323-327.[CrossRef]
    [Google Scholar]
  23. Wibowo, D., Eschenbruch, R., Davis, C. R. & Lee, T. H. ( 1985; ). Occurrence and growth of lactic acid bacteria in wine: a review. Am J Enol Vitic 36, 302-313.
    [Google Scholar]
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