1887

Abstract

A penicillin-resistant mutant, JH2-2r (MIC 75 μg ml), was isolated from JH2-2 (MIC 5 μg ml) by successive passages on plates containing increasing concentrations of benzylpenicillin. A comparison of the penicillin-binding protein (PBP) profiles in the two strains revealed a more intensely labelled PBP4 in JH2-2r. Because the sequences of the JH2-2 and JH2-2r genes were strictly identical, even in their promoter regions, this intensive labelling could only be associated with an overproduction of the low-affinity PBP4. No gene analogous to that proposed to act as a regulator of PBP5 synthesis in and could be identified in the vicinity of in JH2-2 and JH2-2r. However, a -like gene distant from was identified. The cloning and sequencing of that -like gene from both strains indicated that they were identical. It is therefore postulated that the PBP4 overproduction in JH2-2r results from the modification of an as yet unidentified factor.

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2001-09-01
2019-09-21
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403-410.[CrossRef]
    [Google Scholar]
  2. Andersen, P. S., Martinussen, J. & Hammer, K. ( 1996; ). Sequence analysis and identification of the pyrKDbF operon from Lactococcus lactis including a novel gene, pyrK involved in pyrimidine biosynthesis. J Bacteriol 178, 5005-5012.
    [Google Scholar]
  3. Courvalin, P. ( 1994; ). Transfer of antibiotic resistance genes between Gram-positive and Gram-negative bacteria. Antimicrob Agents Chemother 38, 1447-1451.[CrossRef]
    [Google Scholar]
  4. Coyette, J., Ghuysen, J. M. & Fontana, R. ( 1978; ). Solubilization and isolation of the membrane-bound dd-carboxypeptidase of Streptococcus faecalis ATCC 9790. Eur J Biochem 88, 297-305.[CrossRef]
    [Google Scholar]
  5. Coyette, J., Ghuysen, J. M. & Fontana, R. ( 1980; ). The penicillin-binding proteins in Streptococcus faecalis ATCC 9790. Eur J Biochem 110, 445-456.[CrossRef]
    [Google Scholar]
  6. Devereux, J., Haeberli, P. & Smithies, O. ( 1984; ). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12, 387-395.[CrossRef]
    [Google Scholar]
  7. El Kharroubi, A., Jacques, P., Piras, G., Van Beeumen, J., Coyette, J. & Ghuysen, J. M. ( 1991; ). The Enterococcus hirae R40 penicillin-binding protein 5 and the methicillin-resistant Staphylococcus aureus penicillin-binding 2′ are similar. Biochem J 280, 463-469.
    [Google Scholar]
  8. Fontana, R., Cerini, R., Longoni, P., Grossato, A. & Canepari, P. ( 1983; ). Identification of a streptococcal penicillin-binding protein that reacts very slowly with penicillin. J Bacteriol 155, 1343-1350.
    [Google Scholar]
  9. Fontana, R., Grossato, A., Rossi, L., Cheng, Y. R. & Satta, G. ( 1985; ). Transition from resistance to hypersusceptibility to β-lactam antibiotics associated with loss of low affinity PBP in a Streptococcus faecium mutant highly resistant to penicillin. Antimicrob Agents Chemother 26, 678-683.
    [Google Scholar]
  10. Fontana, R., Aldegheri, M., Ligozzi, M., Lopez, H., Sucari, A. & Satta, G. ( 1994; ). Overproduction of a low-affinity penicillin-binding protein and high-level ampicillin resistance in Enterococcus faecium. Antimicrob Agents Chemother 38, 1980-1983.[CrossRef]
    [Google Scholar]
  11. Ghuysen, J. M., Frère, J. M., Leyh-Bouille, M., Nguyen-Distèche, M. & Coyette, J. ( 1986; ). Active-site serine d-alanyl-d-alanine-cleaving peptidase-catalysed acyl-transfer reactions. Procedures for studying the penicillin-binding proteins of bacterial plasma membranes. Biochem J 235, 159-165.
    [Google Scholar]
  12. Goffin, C. & Ghuysen, J. M. ( 1998; ). Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs. Microbiol Mol Biol Rev 62, 1079-1093.
    [Google Scholar]
  13. Griffin, A. M., Morris, V. J. & Gasson, M. J. ( 1996; ). The cpsABCDE genes involved in polysaccharide production in Streptococcus salivarius spp. thermophilus NCBF2393. Gene 183, 23-27.[CrossRef]
    [Google Scholar]
  14. Gutmann, L. (1994). Résistance des entérocoques aux bêta-lactamines et conséquences sur les synergies. Méd Mal Infect (spécial) 24, 165–171.
  15. Hancock, L. E. & Gilmore, M. S. ( 2000; ). Pathogenicity of enterococci. In Gram-positive Pathogens , pp. 251-288. Edited by V. A. Fischetti, R. P. Novick, J. J. Ferretti, D. A. Portnoy & J. I. Road. Washington, DC:ASM Press.
  16. Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J. (1990). PCR protocols. A Guide to Methods and Applications. New York: Academic Press.
  17. Jett, B. D., Huycke, M. M. & Gilmore, M. S. ( 1994; ). Virulence of enterococci. Clin Microbiol Rev 7, 462-478.
    [Google Scholar]
  18. Klare, I., Rodloff, A. C., Wagner, J., Witte, W. & Hakenbeck, R. ( 1992; ). Overproduction of a penicillin-binding protein is not the only mechanism of penicillin resistance in Enterococcus faecium. Antimicrob Agents Chemother 36, 783-787.[CrossRef]
    [Google Scholar]
  19. Kunst, F., Ogasawara, N., Moszer, I. & 148 other authors ( 1997; ). The complete sequence of the Gram-positive bacterium Bacillus subtilis. Nature 390, 249–256.[CrossRef]
    [Google Scholar]
  20. Laskey, R. A. ( 1980; ). The use of intensifying screens or organic scintillators for visualizing radioactive molecules, resolved by gel electrophoresis. Methods Enzymol 65, 363-371.
    [Google Scholar]
  21. Lazarevic, V., Margot, P., Soldo, B. & Karamata, D. ( 1992; ). Sequencing and analysis of the Bacillus subtilis lytRABC divergon: a regulatory unit encompassing the structural genes of the N-acetylmuramoyl-l-alanine amidase and its modifier. J Gen Microbiol 138, 1949-1961.[CrossRef]
    [Google Scholar]
  22. Ligozzi, M., Pittaluga, F. & Fontana, R. ( 1993; ). Identification of a genetic element (psr) which negatively controls expression of Enterococcus hirae penicillin-binding protein 5. J Bacteriol 175, 2046-2051.
    [Google Scholar]
  23. Ligozzi, M., Pittaluga, F. & Fontana, R. ( 1996; ). Modification of the penicillin-binding protein 5 associated with high-level ampicillin resistance of Enterococcus faecium. Antimicrob Agents Chemother 40, 354-357.
    [Google Scholar]
  24. Lillehang, D., Nes, I. F. & Birkeland, N. K. ( 1997; ). A highly efficient and stable system for site-specific integration of genes and plasmids into the phage ϕLC3 attachment site (attB) of the Lactococcus lactis chromosome. Gene 188, 129-136.[CrossRef]
    [Google Scholar]
  25. Lindström, E. B., Boman, H. G. & Steele, B. B. ( 1970; ). Resistance of Escherichia coli to penicillin. VI. Purification and characterization of the chromosomally mediated penicillinase present in ampA-containing strains. J Bacteriol 101, 218-231.
    [Google Scholar]
  26. Loureiro Dos Santos, A. L. & Chopin, A. ( 1987; ). Shotgun cloning in Streptococcus lactis. FEMS Microbiol Lett 42, 209-212.
    [Google Scholar]
  27. Mainardi, J. L., Billot-Klein, D., Coutrot, A., Legrand, R., Schoot, B. & Gutmann, L. ( 1998; ). Resistance to cefotaxime and peptidoglycan composition in Enterococcus faecalis are influenced by exogenous sodium chloride. Microbiology 144, 2679-2685.[CrossRef]
    [Google Scholar]
  28. Massidda, O., Dardenne, O., Whalen, M. B., Zorzi, W., Coyette, J., Shockman, G. D. & Daneo-Moore, L. ( 1998; ). The PBP5 synthesis repressor (psr) gene of Enterococcus hirae ATCC 9790 is substantially longer than previously reported. FEMS Microbiol Lett 166, 355-360.[CrossRef]
    [Google Scholar]
  29. Masson, J. M. & Labia, R. ( 1983; ). Synthesis of a 125I-radiolabeled penicillin for penicillin-binding protein studies. Anal Biochem 128, 164-168.[CrossRef]
    [Google Scholar]
  30. Mollerach, M., Partoune, P., Coyette, J. & Ghuysen, J. M. ( 1996; ). Importance of the E46-D160 polypeptide segment of the non-penicillin-binding module for the stability of the low-affinity, multimodular class B penicillin-binding protein 5 of Enterococcus hirae. J Bacteriol 178, 1774-1775.
    [Google Scholar]
  31. Morona, J. K., Morona, R. & Paton, J. C. ( 1997; ). Characterization of the locus encoding the Streptococcus pneumoniae type 19F capsular polysaccharide biosynthetic pathway. Mol Microbiol 23, 751-753.[CrossRef]
    [Google Scholar]
  32. Murray, B. E. ( 1990; ). The life and times of the Enterococcus. Clin Microbiol Rev 3, 46-65.
    [Google Scholar]
  33. Murray, T., Popham, D. L. & Setlow, P. ( 1996; ). Identification and characterization of pbpC, the gene encoding Bacillus subtilis penicillin-binding protein 3. J Bacteriol 178, 6001-6005.
    [Google Scholar]
  34. Nakano, Y., Yoshida, Y., Yamashita, Y. & Koga, T. ( 1995; ). Construction of a series of pACYC-derived plasmid vectors. Gene 162, 157-158.[CrossRef]
    [Google Scholar]
  35. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence analysis. Proc Natl Acad Sci USA 85, 2444-2448.[CrossRef]
    [Google Scholar]
  36. Piras, G., El Kharroubi, A., Van Beeumen, J., Coeme, E., Coyette, J. & Ghuysen, J. M. ( 1990; ). Characterization of an Enterococcus hirae penicillin-binding protein 3 with low penicillin affinity. J Bacteriol 172, 6856-6862.
    [Google Scholar]
  37. Piras, G., Raze, D., El Kharroubi, A., Hastir, D., Englebert, S., Coyette, J. & Ghuysen, J. M. ( 1993; ). Cloning and sequencing of the low affinity penicillin-binding protein 3r-encoding gene of Enterococcus hirae S185: modular design and structural organization of the protein. J Bacteriol 175, 2844-2852.
    [Google Scholar]
  38. Rice, L. B., Carias, L. L., Hutton-Thomas, R., Sifaoui, F., Gutmann, L. & Rudin, S. D. ( 2001; ). Penicillin-binding protein 5 and expression of ampicillin resistance in Enterococcus faecium. Antimicrob Agents Chemother 45, 1480-1486.[CrossRef]
    [Google Scholar]
  39. Schmit, J. L., Leclercq, R., Scheimberg, A. & Laudaner, D. I. (1994). Approche épidémiologique et clinique des entérocoques: résultats d’une enquête. Méd Mal Infect (spécial) 24, 141–148.
  40. Signoretto, C., Boaretti, M. & Canepari, P. ( 1994; ). Cloning, sequencing and expression in Escherichia coli of the low affinity penicillin-binding protein of Enterococcus faecalis. FEMS Microbiol Lett 123, 99-106.[CrossRef]
    [Google Scholar]
  41. Soldo, B., Lazarevic, V., Mauël, C. & Karamata, D. ( 1996; ). Sequence of the 305°–307° region of the Bacillus subtilis chromosome. Microbiology 142, 3079-3088.[CrossRef]
    [Google Scholar]
  42. Song, M. D., Wachi, M., Doi, M., Ishino, F. & Matsuhashi, M. ( 1987; ). Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus. FEBS Lett 221, 167-171.[CrossRef]
    [Google Scholar]
  43. Stingele, F., Neeser, J. R. & Mollet, B. ( 1996; ). Identification and characterization of the eps (exopolysaccharide) gene cluster from Streptococcus thermophilus Sfi6. J Bacteriol 178, 1680-1690.
    [Google Scholar]
  44. Van Sinderen, D., Karsens, H., Kok, J., Terpstra, P., Ruiters, M. H., Venema, G. & Nautra, A. ( 1996; ). Sequence analysis and molecular characterization of the temperate lactococcal bacteriophage r1t. Mol Microbiol 19, 1343-1355.[CrossRef]
    [Google Scholar]
  45. Williamson, R., Le Bouguénec, C., Gutmann, L. & Horaud, T. ( 1985; ). One or two low-affinity penicillin-binding proteins may be responsible for the range of susceptibility of Enterococcus faecium to benzylpenicillin. J Gen Microbiol 131, 1933-1940.
    [Google Scholar]
  46. Williamson, R., Gutmann, L., Horaud, T., Delbos, F. & Acar, J. F. ( 1986; ). Use of penicillin-binding proteins for identification of enterococci. J Gen Microbiol 132, 1929-1937.
    [Google Scholar]
  47. Zorzi, W., Zhou, X. Y., Dardenne, O., Lamotte, J., Raze, D., Pierre, J., Gutmann, L. & Coyette, J. ( 1996; ). Structure of the low-affinity penicillin-binding protein 5, PBP5fm, in wild-type and highly penicillin-resistant strains of Enterococcus faecium. J Bacteriol 178, 4948-4957.
    [Google Scholar]
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