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Volume 147, Issue 9

HO-nonproducing strains: survival in stationary phase and virulence in chronic granulomatous disease Free

Abstract

The production of hydrogen peroxide (HO) and related phenotypes were studied with strains isolated from cases of pharyngitis or severe group A streptococcal infections. Of the 46 strains examined (34 from severe infections and 12 from pharyngitis cases), 25 strains accumulated HO in the culture medium when grown under glucose-limited, aerobic conditions, whereas the rest of the strains did not. There was no correlation between these traits and the type of disease from which each strain had been isolated. The HO-nonproducing strains tested in this study belonged to T type 3 or T type 12. The accumulation of HO started when the culture reached the late exponential phase. A rapid loss of cell viability accompanied HO accumulation but was completely prevented by the addition of a catalase, indicating that the lethality was actually caused by HO. Cells of HO-nonproducing strains were resistant to killing by phagocytes from patients with chronic granulomatous disease (CGD), whereas those of HO-producing strains were subject to killing. Subcutaneous inoculation of 10 c.f.u. HO-nonproducing strains into the hind footpads of CGD mice provoked more prominent swelling of the footpad than did HO-producing strains. The mortality rate in the CGD mice infected with the HO-nonproducing strains was higher than that produced by the HO-producing strains. It is suggested that HO-nonproducing strains are prevalent in humans and that they may be a potential threat to the health of CGD patients.

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Keyword(s): BHI, brain-heart infusion , CGD , CGD, chronic granulomatous disease , gp91-phox knockout mice , group A streptococci and hydrogen peroxide
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2001-09-01
2023-06-10
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H2O2-nonproducing Streptococcus pyogenes strains: survival in stationary phase and virulence in chronic granulomatous disease
Microbiology 147, 2469 (2001); https://doi.org/10.1099/00221287-147-9-2469
/content/journal/micro/10.1099/00221287-147-9-2469
/content/journal/micro/10.1099/00221287-147-9-2469
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