Energy metabolism of during anaerobic and microaerobic growth in low- and high-potassium continuous culture Free

Abstract

, a member of the gamma subclass of the , has been implicated as the agent responsible for human periodontitis. In this study, 301-b was grown in fructose-limited chemostat cultures under anaerobic [redox potential ( )<−400 mV] and microaerobic ( =−200 mV) conditions to characterize its energy metabolism. Effects of K and Na on growth and metabolism were also examined. In a control medium containing 52 mM K and 24 mM Na, the molar growth yield on fructose ( ) of microaerobic cultures was 13 times higher than the yield of anaerobic cultures at ≤010 h, but the difference in the between microaerobic and anaerobic cultures decreased at ≤010 h. When the ATP yield from fermentation was estimated from the amounts of fructose consumed and acetate formed, the value of the microaerobic culture (249 mol ATP produced per mol fructose consumed) was lower than the anaerobic value [313 mol ATP (mol fructose)]. Therefore, ATP production from fermentation could not account for the increase in the at >010 h and thus additional ATP was expected to be generated via respiration. Assuming that the (g cells formed per mol ATP synthesized) was similar between anaerobic and microaerobic cultures, the estimated ATP yield from respiration was between 12 and 20 mol ATP (mol fructose) below =010 h and decreased to 03 mol ATP (mol fructose) when was increased to 019 h. Such growth-rate-dependent decreases in the and the estimated ATP production from respiration were also observed in a high-Na (52 mM K and 106 mM Na) culture but not in a high-K (81 mM K and 24 mM Na) culture. In the high-K culture, the microaerobic was 14–20 times higher than the anaerobic value and the respiration-derived ATP yield was estimated to be between 12 and 19 mol ATP (mol fructose) over a wide range of dilution rate. These results suggest that higher concentrations of extracellular K are required for the respiration to occur in rapidly growing cells of

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-147-9-2461
2001-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/147/9/1472461a.html?itemId=/content/journal/micro/10.1099/00221287-147-9-2461&mimeType=html&fmt=ahah

References

  1. Bakker E. P. 1993 Alkali Cation Transport Systems in Prokaryotes Boca Raton, FL: CRC Press, Inc;
    [Google Scholar]
  2. Bakker E. P., Booth I. R., Dinnbier U., Epstein W., Gajewska A. 1987; Evidence for multiple K+ export systems in Escherichia coli . J Bacteriol 169:3743–3749
    [Google Scholar]
  3. Bang J., Cimasoni G., Rosenbusch C., Duckert A. 1973; Sodium, potassium and calcium contents of crevicular exudate: their relations to gingivitis and periodontitis. J Periodontol 44:770–774 [CrossRef]
    [Google Scholar]
  4. Dzink J. L., Tanner A. C. R., Haffajee A. D., Socransky S. S. 1985; Gram negative species associated with active destructive periodontal lesions. J Clin Periodontol 12:648–659 [CrossRef]
    [Google Scholar]
  5. Epstein W., Burrman E., McLaggan D., Naprstek J. 1993; Multiple mechanisms, roles and controls of K+ transport in Escherichia coli . Biochem Soc Trans 21:1006–1010
    [Google Scholar]
  6. Fleischmann R. D., Adams M. D., White O. 37 other authors 1995; Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496–512 [CrossRef]
    [Google Scholar]
  7. Hueting S., de Lange T., Tempest D. W. 1979; Energy requirement for maintenance of the transmembrane potassium gradient in Klebsiella aerogenes NCTC 418: a continuous culture study. Arch Microbiol 123:183–188 [CrossRef]
    [Google Scholar]
  8. Inouye T., Ohta H., Kokeguchi K., Fukui K., Kato K. 1990; Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans . FEMS Microbiol Lett 69:13–18 [CrossRef]
    [Google Scholar]
  9. Kakinuma Y. 1998; Inorganic cation transport and energy transduction in Enterococcus hirae and other streptococci. Microbiol Mol Biol Rev 62:1021–1045
    [Google Scholar]
  10. Kielley W. W. 1963; Preparation and assay of phosphorylating submitochondrial particles: sonicated mitochondria. Methods Enzymol 6:272–277
    [Google Scholar]
  11. Loesche W. J., Gusberti F., Mettraux G., Higgins T., Syed S. 1983; Relationship between oxygen tension and subgingival bacterial flora in untreated human periodontal pockets. Infect Immun 42:659–667
    [Google Scholar]
  12. Mannheim W., Stieler W., Wolf G., Zabel R. 1978; Taxonomic significance of respiratory quinones and fumarate respiration in Actinobacillus and Pasteurella. Int J Syst Bacteriol 28:7–13 [CrossRef]
    [Google Scholar]
  13. Ohta H., Gottschal J. C. 1988; Microaerophilic growth of Wolinella recta ATCC 33238. FEMS Microbiol Ecol 53:79–86 [CrossRef]
    [Google Scholar]
  14. Ohta H., Taniguchi S. 1988; Respiratory characteristics of two oligotrophic bacteria: Agromonas oligotrophica JCM 1494 and Aeromonas hydrophila 315. J Gen Appl Microbiol 34:355–365 [CrossRef]
    [Google Scholar]
  15. Ohta H., Kokeguchi S., Fukui K., Kato K. 1987; Leukotoxic activity in Actinobacillus ( Haemophilus ) actinomycetemcomitans isolated from periodontal disease patients. Microbiol Immunol 31:313–325 [CrossRef]
    [Google Scholar]
  16. Ohta H., Fukui K., Kato K. 1989; Effect of bicarbonate on the growth of Actinobacillus actinomycetemcomitans in anaerobic fructose-limited chemostat cultures . J Gen Microbiol 135:3485–3495
    [Google Scholar]
  17. Ohta H., Miyagi A., Kato K., Fukui K. 1996a; The relationships between leukotoxin production, growth rate and the bicarbonate concentration in a toxin-production-variable strain of Actinobacillus actinomycetemcomitans. Microbiology 142:963–970 [CrossRef]
    [Google Scholar]
  18. Ohta H., Moriki D., Miyagi A., Watanabe T., Kato K., Fukui K. 1996b; Microaerophilic property of Actinobacillus actinomycetemcomitans in fructose-limited chemostat cultures. FEMS Microbiol Lett 136:191–196
    [Google Scholar]
  19. Silver S. 1978; Transport of cations and anions. In Bacterial Transport pp 221–234 Edited by Rosen B. P. New York: Marcel Dekker;
    [Google Scholar]
  20. Slots J., Schonfeld S. E. 1991; Actinobacillus actinomycetemcomitans in localized juvenile periodontitis. In Periodontal Disease: Pathogens and Host Immune Responses pp 53–64 Edited by Hamada S., Holt S. C., McGhee J. R. Tokyo: Quintessence Publishing;
    [Google Scholar]
  21. Slots J., Reynolds H. S., Genco R. J. 1980; Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun 29:1013–1020
    [Google Scholar]
  22. Stouthamer A. H., Bettenhaussen C. W. 1975; Determination of the efficiency of oxidative phosphorylation in continuous cultures of Aerobacter aerogenes. Arch Microbiol 102:187–192 [CrossRef]
    [Google Scholar]
  23. Tatevossian A., Gould C. T. 1976; The composition of the aqueous phase in human dental plaque. Arch Oral Biol 21:319–323 [CrossRef]
    [Google Scholar]
  24. Tempest D. W., Neijssel O. M. 1984; The status of Y ATP and maintenance energy as biologically interpretable phenomena. Annu Rev Microbiol 38:459–486 [CrossRef]
    [Google Scholar]
  25. Tempest D. W., Dicks J. W., Hunter J. R. 1966; The inter-relationship between potassium, magnesium and phosphorus in potassium-limited chemostat cultures of Aerobacter aerogenes. J Gen Microbiol 45:135–146 [CrossRef]
    [Google Scholar]
  26. Wilson M., Henderson B. 1995; Virulence factors of Actinobacillus actinomycetemcomitans relevant to the pathogenesis of inflammatory periodontal disease. FEMS Microbiol Rev 17:365–379 [CrossRef]
    [Google Scholar]
  27. Zambon J. 1985; Actinobacillus actinomycetemcomitans in human periodontal disease. J Clin Periodontol 12:1–20 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-147-9-2461
Loading
/content/journal/micro/10.1099/00221287-147-9-2461
Loading

Data & Media loading...

Most cited Most Cited RSS feed