1887

Abstract

, a member of the gamma subclass of the , has been implicated as the agent responsible for human periodontitis. In this study, 301-b was grown in fructose-limited chemostat cultures under anaerobic [redox potential ( )<−400 mV] and microaerobic ( =−200 mV) conditions to characterize its energy metabolism. Effects of K and Na on growth and metabolism were also examined. In a control medium containing 52 mM K and 24 mM Na, the molar growth yield on fructose ( ) of microaerobic cultures was 13 times higher than the yield of anaerobic cultures at ≤010 h, but the difference in the between microaerobic and anaerobic cultures decreased at ≤010 h. When the ATP yield from fermentation was estimated from the amounts of fructose consumed and acetate formed, the value of the microaerobic culture (249 mol ATP produced per mol fructose consumed) was lower than the anaerobic value [313 mol ATP (mol fructose)]. Therefore, ATP production from fermentation could not account for the increase in the at >010 h and thus additional ATP was expected to be generated via respiration. Assuming that the (g cells formed per mol ATP synthesized) was similar between anaerobic and microaerobic cultures, the estimated ATP yield from respiration was between 12 and 20 mol ATP (mol fructose) below =010 h and decreased to 03 mol ATP (mol fructose) when was increased to 019 h. Such growth-rate-dependent decreases in the and the estimated ATP production from respiration were also observed in a high-Na (52 mM K and 106 mM Na) culture but not in a high-K (81 mM K and 24 mM Na) culture. In the high-K culture, the microaerobic was 14–20 times higher than the anaerobic value and the respiration-derived ATP yield was estimated to be between 12 and 19 mol ATP (mol fructose) over a wide range of dilution rate. These results suggest that higher concentrations of extracellular K are required for the respiration to occur in rapidly growing cells of

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2001-09-01
2019-10-19
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