1887

Abstract

The fermentative lactate dehydrogenase (LDH) of is induced by low pH under anaerobic conditions. Both translational and transcriptional gene fusions to , which encodes the fermentative LDH, have now been made. Both types of fusion were induced by low pH, but only in the absence of air. However, the translational fusions were consistently expressed at a five- to tenfold higher level than the transcriptional fusions, perhaps implying some post-transcriptional effect on expression. Introduction of ::Kan decreased expression of both translational and transcriptional fusions by three- to fivefold. Disruption of , which encodes a repressor of several genes of the phosphotransferase system, almost abolished expression of . Disruption of caused a moderate drop in expression of both operon and protein fusions, whereas insertional inactivation of or had the opposite effect. These effects are probably indirect, resulting from alterations in sugar accumulation versus storage. Mutations in , , , , , , , /, , and had no effect on expression of the fusions. was not induced by the membrane-permeant weak acid benzoate, implying that it does not respond to the internal pH directly. Little pH induction was seen during growth on glycerol plus fumarate, suggesting that products of sugar fermentation are necessary for acid induction. Addition of succinate, acetate or lactate had no effect on expression. In contrast, pyruvate caused a two- to fourfold increase in expression of . This accords with the idea that increased sugar metabolism indirectly induces .

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2001-09-01
2019-12-12
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